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mmu_circAcacb_432
Basic information of circular RNA
PanCircBase ID:
mmu_circAcacb_432
mm39_Chr. Position ID:
chr5|114341450|114345431|+
Gene name:
Acacb
Transcript:
ENSMUST00000102582.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
17,18,19
Exon/intron offsets:
0,1282,3846
Genomic length:
3981
Exon/intron sizes in nt:
14,61,51,135
Flanking exon/introns:
chr5:114340041-114341450|chr5:114345431-114347813
Splice length:
432
Other database IDs:
CIRCpedia: MMU_CIRCpedia_39644, circAtlas: mmu-Acacb_0007
circAtlas: hsa-ACACB_0042, CIRCpedia: HSA_CIRCpedia_173826
Human Ortholog IDs:
Mature spliced Sequence:
GTAGCCCGACAGTCCCTGACTATGTTTGTCCTCATCATGAACGGCTGCCACATCGAGATCGATGCCCACCGGCTGAACGATGGGGGGCTGCTCCTGTCCTACAATGGCAGCAGTTACACTACGTACATGAAGGAAGAGGTTGACAGTTACCGGATCACTATCGGCAATAAGACTTGCGTGTTTGAAAAGGAAAACGACCCCACTGTCCTGAGATCCCCCTCGGCTGGGAAGCTGATGCAGTATACGGTGGAGGACGGAGACCACGTGGAAGCTGGGAGCAGCTACGCGGAGATGGAGGTGATGAAGATGATCATGACCCTGAACGTGCAGGAGAGCGGCCGGGTGAAGTACATCAAGCGGCCAGGGGTTATACTGGAGGCTGGCTGCGTGGTGGCGAGGCTAGAACTCGATGACCCTTCAAAAGTGCACGCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCGGGTGAAGTACATCAA
60.04
ATGTGGCAGCCGTTCATGAT
60.39
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCGGGTGAAGTACATCAA
60.04
GCAGCCGTTCATGATGAGGA
60.46
144
PCR template sequence:
GAGCGGCCGGGTGAAGTACATCAAGCGGCCAGGGGTTATACTGGAGGCTGGCTGCGTGGTGGCGAGGCTAGAACTCGATGACCCTTCAAAAGTGCACGCGGTAGCCCGACAGTCCCTGACTATGTTTGTCCTCATCATGAACGGCTGCCACATCGAGATCGATGCCCACCGGCTGAACGATGGGGGGCTGCTCCTGTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-433-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAAGTGCACGCGGTAGCCCGACAGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACACB_432_ORF_1
1.3041
0.56685575
0.996176836
843
ORF sequence:
ATGTTTGTCCTCATCATGAACGGCTGCCACATCGAGATCGATGCCCACCGGCTGAACGATGGGGGGCTGCTCCTGTCCTACAATGGCAGCAGTTACACTACGTACATGAAGGAAGAGGTTGACAGTTACCGGATCACTATCGGCAATAAGACTTGCGTGTTTGAAAAGGAAAACGACCCCACTGTCCTGAGATCCCCCTCGGCTGGGAAGCTGATGCAGTATACGGTGGAGGACGGAGACCACGTGGAAGCTGGGAGCAGCTACGCGGAGATGGAGGTGATGAAGATGATCATGACCCTGAACGTGCAGGAGAGCGGCCGGGTGAAGTACATCAAGCGGCCAGGGGTTATACTGGAGGCTGGCTGCGTGGTGGCGAGGCTAGAACTCGATGACCCTTCAAAAGTGCACGCGGTAGCCCGACAGTCCCTGACTATGTTTGTCCTCATCATGAACGGCTGCCACATCGAGATCGATGCCCACCGGCTGAACGATGGGGGGCTGCTCCTGTCCTACAATGGCAGCAGTTACACTACGTACATGAAGGAAGAGGTTGACAGTTACCGGATCACTATCGGCAATAAGACTTGCGTGTTTGAAAAGGAAAACGACCCCACTGTCCTGAGATCCCCCTCGGCTGGGAAGCTGATGCAGTATACGGTGGAGGACGGAGACCACGTGGAAGCTGGGAGCAGCTACGCGGAGATGGAGGTGATGAAGATGATCATGACCCTGAACGTGCAGGAGAGCGGCCGGGTGAAGTACATCAAGCGGCCAGGGGTTATACTGGAGGCTGGCTGCGTGGTGGCGAGGCTAGAACTCGATGACCCTTCAAAAGTGCACGCG
Peptide sequence:
MFVLIMNGCHIEIDAHRLNDGGLLLSYNGSSYTTYMKEEVDSYRITIGNKTCVFEKENDPTVLRSPSAGKLMQYTVEDGDHVEAGSSYAEMEVMKMIMTLNVQESGRVKYIKRPGVILEAGCVVARLELDDPSKVHAVARQSLTMFVLIMNGCHIEIDAHRLNDGGLLLSYNGSSYTTYMKEEVDSYRITIGNKTCVFEKENDPTVLRSPSAGKLMQYTVEDGDHVEAGSSYAEMEVMKMIMTLNVQESGRVKYIKRPGVILEAGCVVARLELDDPSKVHA
IRESfinder result
IRESfinder score
0.119652705
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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