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mmu_circAbl2_1842

Basic information of circular RNA
PanCircBase ID:

mmu_circAbl2_1842

mm39_Chr. Position ID:

chr1|156450054|156468799|+

Gene name:

Abl2

Transcript:

ENSMUST00000166172.9

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

10

Exon/intron indexes:

3,4,5,6,7,8,9,10,11,12

Exon/intron offsets:

0,2803,7368,8860,10595,11268,12580,14333,17954,18508

Genomic length:

18745

Exon/intron sizes in nt:

1,71,29,62,73,85,17,81,00,00,00,00,00,000

Flanking exon/introns:

chr1:156448471-156450054|chr1:156468799-156469111

Splice length:

1842

Other database IDs:

CIRCpedia: MMU_CIRCpedia_225265, circAtlas: mmu-Abl2_0013

circAtlas: hsa-ABL2_0022, CIRCpedia: HSA_CIRCpedia_69241

Human Ortholog IDs:
Mature spliced Sequence:

AAGTTTTGCACCGCCCGTTTGGCTGTGATGCTGAATCTCAGGCACTGAATGAAGCGATCAGGTGGAGCTCCAAGGAGAACTTGCTGGGAGCCACTGAGAGTGACCCTAATCTCTTTGTTGCACTTTATGACTTTGTGGCAAGTGGTGATAACACACTCAGTATCACTAAAGGTGAAAAGCTGCGAGTCCTTGGTTATAACCAGAATGGCGAGTGGAGTGAAGTTCGCTCCAAGAATGGACAGGGTTGGGTGCCAAGCAACTACATCACTCCAGTTAATAGCCTGGAGAAACATTCCTGGTACCACGGACCTGTATCCCGCAGCGCAGCAGAGTATCTCCTCAGCAGCCTAATCAATGGCAGCTTCCTGGTTCGAGAGAGTGAGAGCAGCCCTGGGCAGCTGTCCATCTCTCTCAGGTATGAGGGACGTGTGTATCACTACAGGATCAATACCACCACAGACAGCAAGGTGTACGTGACAGCTGAGAGCCGCTTTAGCACCTTGGCAGAGCTTGTTCACCACCACTCCACAGTTGCTGATGGGCTAGTGACCACGCTGCACTACCCAGCACCGAAGTGCAACAAGCCAACCGTCTATGGCGTGTCTCCTATCCATGACAAGTGGGAAATGGAACGGACAGATATCACCATGAAGCACAAACTTGGGGGCGGTCAGTACGGAGAGGTTTACGTTGGCGTCTGGAAGAAGTACAGCCTTACAGTGGCTGTGAAAACACTGAAGGAAGACACCATGGAGGTGGAGGAGTTCCTGAAGGAAGCTGCAGTGATGAAGGAGATCAAGCATCCTAACTTAGTACAACTGCTAGGTGTGTGTACCCTGGAGCCACCGTTTTACATTGTGACTGAATACATGCCGTATGGGAACCTGCTTGACTATCTCCGGGAGTGCAGCCGAGAGGAGGTGACCGCCGTCGTGTTACTTTACATGGCCACCCAGATCTCCTCTGCCATGGAGTACTTGGAGAAGAAGAACTTCATCCATAGAGATCTTGCTGCCCGGAACTGCCTAGTGGGCGAGAACCATGTGGTGAAAGTGGCCGACTTTGGTTTAAGTAGACTGATGACTGGAGATACCTACACTGCTCATGCTGGAGCCAAATTTCCTATTAAATGGACAGCACCCGAGAGTCTGGCCTACAATACCTTTTCAATTAAATCTGACGTTTGGGCGTTTGGAGTACTGTTGTGGGAAATTGCTACATATGGAATGTCACCATATCCAGGTATTGACCTATCTCAAGTCTATGACCTACTGGAAAAAGGATATCGAATGGAACAGCCTGAGGGATGCCCCCCTAAAGTGTATGAACTTATGAGAGCATGCTGGAAGTGGAGCCCTGCTGACAGGCCCTCTTTTGCTGAAACCCATCAAGCTTTTGAAACAATGTTCCATGACTCCAGCATCTCTGAAGAGGTAGCTGAGGAGCTTGGGAGAACGGCCTCCTCCTCCTCTGTGGTTCCATACCTGCCTCGGTTACCGCTGCTTCCTTCCAAGACGAGGACCCTGAGGAAGCAGGGGGAGAACAAAGAGAACCTAGATGGGGGCCTTGATGCCGCCGAGAGCCTGGCCTCCAGCTCAGCACCAGCAGGGTTCATTAGAAGCACACAGGCCTCCAGTGGGTCCCCAGCTCTGCCTCGAAAGCAAAGAGATAAGTCGCCCAGCAGCCTCTTAGAAGATGCCAAAGAGACATGCTTTACCAGGGATAGGAAGGGGGGCTTCTTCAGCTCCTTCATGAAAAAGAGGAACGCCCCCACGCCCCCGAAGCGCAGCAGCTCCTTTCGAGAAATGGAGAATCAGCCCCACAAGAAATATGAACTCACGG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GAAAAAGAGGAACGCCCCCA

60.54

GCTCCCAGCAAGTTCTCCTT

59.96

180

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCAGCAGCTCCTTTCGAGAA

60.67

GCTCCCAGCAAGTTCTCCTT

59.96

148

PCR template sequence:

AGCTCCTTCATGAAAAAGAGGAACGCCCCCACGCCCCCGAAGCGCAGCAGCTCCTTTCGAGAAATGGAGAATCAGCCCCACAAGAAATATGAACTCACGGAAGTTTTGCACCGCCCGTTTGGCTGTGATGCTGAATCTCAGGCACTGAATGAAGCGATCAGGTGGAGCTCCAAGGAGAACTTGCTGGGAGCCACTGAGAG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

23

Interacting miRNAs (#binding sites)

mmu-miR-298-5p(2), mmu-miR-106a-5p(1), mmu-miR-17-5p(1), mmu-miR-150-5p(1), mmu-miR-34c-5p(1), mmu-miR-26a-5p(1), mmu-miR-205-5p(1), mmu-miR-106b-5p(1), mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-138-5p(1), mmu-miR-339-5p(1), mmu-miR-503-5p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-96-5p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-370-3p(1), mmu-miR-680(1), mmu-miR-207(1), mmu-miR-188-3p(1), mmu-miR-338-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TATGAACTCACGGAAGTTTTGCACCG

siRNA sets

AAAACTTCCGTGAGTTCAT, CAAAACTTCCGTGAGTTCA, AAACTTCCGTGAGTTCATA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCABL2_1842_ORF_1

1.0236

0.290032689

0.999999998

3057

ORF sequence:

ATGGAACGGACAGATATCACCATGAAGCACAAACTTGGGGGCGGTCAGTACGGAGAGGTTTACGTTGGCGTCTGGAAGAAGTACAGCCTTACAGTGGCTGTGAAAACACTGAAGGAAGACACCATGGAGGTGGAGGAGTTCCTGAAGGAAGCTGCAGTGATGAAGGAGATCAAGCATCCTAACTTAGTACAACTGCTAGGTGTGTGTACCCTGGAGCCACCGTTTTACATTGTGACTGAATACATGCCGTATGGGAACCTGCTTGACTATCTCCGGGAGTGCAGCCGAGAGGAGGTGACCGCCGTCGTGTTACTTTACATGGCCACCCAGATCTCCTCTGCCATGGAGTACTTGGAGAAGAAGAACTTCATCCATAGAGATCTTGCTGCCCGGAACTGCCTAGTGGGCGAGAACCATGTGGTGAAAGTGGCCGACTTTGGTTTAAGTAGACTGATGACTGGAGATACCTACACTGCTCATGCTGGAGCCAAATTTCCTATTAAATGGACAGCACCCGAGAGTCTGGCCTACAATACCTTTTCAATTAAATCTGACGTTTGGGCGTTTGGAGTACTGTTGTGGGAAATTGCTACATATGGAATGTCACCATATCCAGGTATTGACCTATCTCAAGTCTATGACCTACTGGAAAAAGGATATCGAATGGAACAGCCTGAGGGATGCCCCCCTAAAGTGTATGAACTTATGAGAGCATGCTGGAAGTGGAGCCCTGCTGACAGGCCCTCTTTTGCTGAAACCCATCAAGCTTTTGAAACAATGTTCCATGACTCCAGCATCTCTGAAGAGGTAGCTGAGGAGCTTGGGAGAACGGCCTCCTCCTCCTCTGTGGTTCCATACCTGCCTCGGTTACCGCTGCTTCCTTCCAAGACGAGGACCCTGAGGAAGCAGGGGGAGAACAAAGAGAACCTAGATGGGGGCCTTGATGCCGCCGAGAGCCTGGCCTCCAGCTCAGCACCAGCAGGGTTCATTAGAAGCACACAGGCCTCCAGTGGGTCCCCAGCTCTGCCTCGAAAGCAAAGAGATAAGTCGCCCAGCAGCCTCTTAGAAGATGCCAAAGAGACATGCTTTACCAGGGATAGGAAGGGGGGCTTCTTCAGCTCCTTCATGAAAAAGAGGAACGCCCCCACGCCCCCGAAGCGCAGCAGCTCCTTTCGAGAAATGGAGAATCAGCCCCACAAGAAATATGAACTCACGGAAGTTTTGCACCGCCCGTTTGGCTGTGATGCTGAATCTCAGGCACTGAATGAAGCGATCAGGTGGAGCTCCAAGGAGAACTTGCTGGGAGCCACTGAGAGTGACCCTAATCTCTTTGTTGCACTTTATGACTTTGTGGCAAGTGGTGATAACACACTCAGTATCACTAAAGGTGAAAAGCTGCGAGTCCTTGGTTATAACCAGAATGGCGAGTGGAGTGAAGTTCGCTCCAAGAATGGACAGGGTTGGGTGCCAAGCAACTACATCACTCCAGTTAATAGCCTGGAGAAACATTCCTGGTACCACGGACCTGTATCCCGCAGCGCAGCAGAGTATCTCCTCAGCAGCCTAATCAATGGCAGCTTCCTGGTTCGAGAGAGTGAGAGCAGCCCTGGGCAGCTGTCCATCTCTCTCAGGTATGAGGGACGTGTGTATCACTACAGGATCAATACCACCACAGACAGCAAGGTGTACGTGACAGCTGAGAGCCGCTTTAGCACCTTGGCAGAGCTTGTTCACCACCACTCCACAGTTGCTGATGGGCTAGTGACCACGCTGCACTACCCAGCACCGAAGTGCAACAAGCCAACCGTCTATGGCGTGTCTCCTATCCATGACAAGTGGGAAATGGAACGGACAGATATCACCATGAAGCACAAACTTGGGGGCGGTCAGTACGGAGAGGTTTACGTTGGCGTCTGGAAGAAGTACAGCCTTACAGTGGCTGTGAAAACACTGAAGGAAGACACCATGGAGGTGGAGGAGTTCCTGAAGGAAGCTGCAGTGATGAAGGAGATCAAGCATCCTAACTTAGTACAACTGCTAGGTGTGTGTACCCTGGAGCCACCGTTTTACATTGTGACTGAATACATGCCGTATGGGAACCTGCTTGACTATCTCCGGGAGTGCAGCCGAGAGGAGGTGACCGCCGTCGTGTTACTTTACATGGCCACCCAGATCTCCTCTGCCATGGAGTACTTGGAGAAGAAGAACTTCATCCATAGAGATCTTGCTGCCCGGAACTGCCTAGTGGGCGAGAACCATGTGGTGAAAGTGGCCGACTTTGGTTTAAGTAGACTGATGACTGGAGATACCTACACTGCTCATGCTGGAGCCAAATTTCCTATTAAATGGACAGCACCCGAGAGTCTGGCCTACAATACCTTTTCAATTAAATCTGACGTTTGGGCGTTTGGAGTACTGTTGTGGGAAATTGCTACATATGGAATGTCACCATATCCAGGTATTGACCTATCTCAAGTCTATGACCTACTGGAAAAAGGATATCGAATGGAACAGCCTGAGGGATGCCCCCCTAAAGTGTATGAACTTATGAGAGCATGCTGGAAGTGGAGCCCTGCTGACAGGCCCTCTTTTGCTGAAACCCATCAAGCTTTTGAAACAATGTTCCATGACTCCAGCATCTCTGAAGAGGTAGCTGAGGAGCTTGGGAGAACGGCCTCCTCCTCCTCTGTGGTTCCATACCTGCCTCGGTTACCGCTGCTTCCTTCCAAGACGAGGACCCTGAGGAAGCAGGGGGAGAACAAAGAGAACCTAGATGGGGGCCTTGATGCCGCCGAGAGCCTGGCCTCCAGCTCAGCACCAGCAGGGTTCATTAGAAGCACACAGGCCTCCAGTGGGTCCCCAGCTCTGCCTCGAAAGCAAAGAGATAAGTCGCCCAGCAGCCTCTTAGAAGATGCCAAAGAGACATGCTTTACCAGGGATAGGAAGGGGGGCTTCTTCAGCTCCTTCATGAAAAAGAGGAACGCCCCCACGCCCCCGAAGCGCAGCAGCTCCTTTCGAGAAATGGAGAATCAGCCCCACAAGAAATATGAACTCACG

Peptide sequence:

MERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECSREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRTASSSSVVPYLPRLPLLPSKTRTLRKQGENKENLDGGLDAAESLASSSAPAGFIRSTQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELTEVLHRPFGCDAESQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTTTDSKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECSREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRTASSSSVVPYLPRLPLLPSKTRTLRKQGENKENLDGGLDAAESLASSSAPAGFIRSTQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELT

IRESfinder result

IRESfinder score

0.438310545

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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