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mmu_circAbi2_529
Basic information of circular RNA
PanCircBase ID:
mmu_circAbi2_529
mm39_Chr. Position ID:
chr1|60483143|60492921|+
Gene name:
Abi2
Transcript:
ENSMUST00000052332.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,3275,4093,9619
Genomic length:
9778
Exon/intron sizes in nt:
98,147,125,159
Flanking exon/introns:
chr1:60476391-60483143|chr1:60492921-60509824
Splice length:
529
Other database IDs:
circBase: mmu_circ_0008855, CIRCpedia: MMU_CIRCpedia_24893, circAtlas: mmu-Abi2_0003
circBase: hsa_circ_0118829, circAtlas: hsa-ABI2_0003, CIRCpedia: HSA_CIRCpedia_39313
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTACCCAGAATATGAAAATGGGTGGATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTCAGGGAAGGGGACACTTGGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAACTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAGCAGTCGGGAGAACAGCGGAAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGCGTCTTTCCAGGTCATCCTGTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCATACACCACCTACAATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCGTTGCCCTATAGACGT
59.62
ACTTGGGGGCTTCTGAGTTG
59.89
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCGTTGCCCTATAGACGT
59.62
AGTTGGAGGTGTCGTACGTG
59.69
147
PCR template sequence:
ATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAGGTGAGTACCCAGAATATGAAAATGGGTGGATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTCAGGGAAGGGGACACTTGGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AACCAGAATCCAGGTGAGTACCCAGA
siRNA sets
GTACTCACCTGGATTCTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCABI2_529_ORF_1
0.9436
0.137644438
0.867312624
669
ORF sequence:
ATGAAAATGGGTGGATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTCAGGGAAGGGGACACTTGGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAACTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAGCAGTCGGGAGAACAGCGGAAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGCGTCTTTCCAGGTCATCCTGTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCATACACCACCTACAATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAGGTGAGTACCCAGAATATGAAAATGGGTGGATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTCAGGGAAGGGGACACTTGGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGA
Peptide sequence:
MKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYNQNPGEYPEYENGWIATYDTSNSEAPKSPYVREGDTWAALPLSNTGASASSSGTK
IRESfinder result
IRESfinder score
0.622850298
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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