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mmu_circAbcc8_863

Basic information of circular RNA
PanCircBase ID:

mmu_circAbcc8_863

mm39_Chr. Position ID:

chr7|45815342|45828007|-

Gene name:

Abcc8

Transcript:

ENSMUST00000033123.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

5

Exon/intron indexes:

6,5,4,3,2

Exon/intron offsets:

0,974,3905,9441,12523

Genomic length:

12665

Exon/intron sizes in nt:

18,92,43,16,71,22,142

Flanking exon/introns:

chr7:45806695-45815342|chr7:45828007-45829225

Splice length:

863

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GATGGGGCAGCCAGAGCTCCAAGGTACACATTCACCATAGCACCTGGCTCCATTTCCCTGGCCACAACCTGCGCTGGATCCTTACCTTCATGCTGCTCTTTGTCCTGGTGTGTGAGATCGCTGAGGGTATCCTGTCTGATGGGGTGACAGAATCCCGCCACCTCCATTTGTACATGCCAGCTGGGATGGCTTTCATGGCTGCCATCACCTCTGTGGTCTACTATCACAACATTGAGACCTCTAACTTCCCCAAGCTGCTGATCGCTCTGCTTATCTACTGGACCTTGGCCTTCATCACTAAGACCATCAAGTTCGTCAAGTTCTACGACCACGCCATCGGCTTCTCTCAGCTGCGCTTCTGCCTCACGGGGCTTCTGGTGATCCTCTACGGGATGCTGCTGCTGGTGGAGATCAATGTCATCCGGGTGCGGAGGTACATCTTCTTCAAGACGCCAAGGGAAGTGAAGCCCCCTGAGGACCTGCAGGACCTGGGGGTGCGCTTTCTGCAGCCCTTCGTGAATCTGCTGTCAAAGGGGACCTACTGGTGGATGAATGCCTTCATCAAGACTGCCCACAGGAAGCCCATCGACCTGCGGGCCATCGGGAAGCTGCCCATCGCCATGAGAGCCCTCACCAACTATCAGCGACTCTGCGCGGCCTTCGATGCCCAGGCGCGGAAAGACACGCAGAGCCAGCAGGGCGCCCGGGCCATCTGGAGGGCTCTCTGCCATGCCTTTGGGAGACGCCTGGTCCTCAGCAGCACATTCCGTATCCTGGCTGACCTGCTGGGCTTTGCTGGACCGCTCTGCATCTTTGGGATCGTGGACCACCTCGGGAAGGAGAACCATGTCTTCCAGCCCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTATCCTGGCTGACCTGCTG

60.18

GTAAGGATCCAGCGCAGGTT

60.11

180

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GTATCCTGGCTGACCTGCTG

60.18

TAAGGATCCAGCGCAGGTTG

60.11

179

PCR template sequence:

ATTCCGTATCCTGGCTGACCTGCTGGGCTTTGCTGGACCGCTCTGCATCTTTGGGATCGTGGACCACCTCGGGAAGGAGAACCATGTCTTCCAGCCCAAGGATGGGGCAGCCAGAGCTCCAAGGTACACATTCACCATAGCACCTGGCTCCATTTCCCTGGCCACAACCTGCGCTGGATCCTTACCTTCATGCTGCTCTT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

11

Interacting miRNAs (#binding sites)

mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-15b-5p(1), mmu-miR-7a-5p(1), mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-214-3p(1), mmu-miR-149-5p(1), mmu-miR-431-5p(1), mmu-miR-107-3p(1), mmu-miR-377-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CTTCCAGCCCAAGGATGGGGCAGCCA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCABCC8_863_ORF_1

1.297

0.422453865

0.99593791

921

ORF sequence:

ATGCTGCTCTTTGTCCTGGTGTGTGAGATCGCTGAGGGTATCCTGTCTGATGGGGTGACAGAATCCCGCCACCTCCATTTGTACATGCCAGCTGGGATGGCTTTCATGGCTGCCATCACCTCTGTGGTCTACTATCACAACATTGAGACCTCTAACTTCCCCAAGCTGCTGATCGCTCTGCTTATCTACTGGACCTTGGCCTTCATCACTAAGACCATCAAGTTCGTCAAGTTCTACGACCACGCCATCGGCTTCTCTCAGCTGCGCTTCTGCCTCACGGGGCTTCTGGTGATCCTCTACGGGATGCTGCTGCTGGTGGAGATCAATGTCATCCGGGTGCGGAGGTACATCTTCTTCAAGACGCCAAGGGAAGTGAAGCCCCCTGAGGACCTGCAGGACCTGGGGGTGCGCTTTCTGCAGCCCTTCGTGAATCTGCTGTCAAAGGGGACCTACTGGTGGATGAATGCCTTCATCAAGACTGCCCACAGGAAGCCCATCGACCTGCGGGCCATCGGGAAGCTGCCCATCGCCATGAGAGCCCTCACCAACTATCAGCGACTCTGCGCGGCCTTCGATGCCCAGGCGCGGAAAGACACGCAGAGCCAGCAGGGCGCCCGGGCCATCTGGAGGGCTCTCTGCCATGCCTTTGGGAGACGCCTGGTCCTCAGCAGCACATTCCGTATCCTGGCTGACCTGCTGGGCTTTGCTGGACCGCTCTGCATCTTTGGGATCGTGGACCACCTCGGGAAGGAGAACCATGTCTTCCAGCCCAAGGATGGGGCAGCCAGAGCTCCAAGGTACACATTCACCATAGCACCTGGCTCCATTTCCCTGGCCACAACCTGCGCTGGATCCTTACCTTCATGCTGCTCTTTGTCCTGGTGTGTGAGATCGCTGAGGGTATCCTGTCTGATGGGGTGA

Peptide sequence:

MLLFVLVCEIAEGILSDGVTESRHLHLYMPAGMAFMAAITSVVYYHNIETSNFPKLLIALLIYWTLAFITKTIKFVKFYDHAIGFSQLRFCLTGLLVILYGMLLLVEINVIRVRRYIFFKTPREVKPPEDLQDLGVRFLQPFVNLLSKGTYWWMNAFIKTAHRKPIDLRAIGKLPIAMRALTNYQRLCAAFDAQARKDTQSQQGARAIWRALCHAFGRRLVLSSTFRILADLLGFAGPLCIFGIVDHLGKENHVFQPKDGAARAPRYTFTIAPGSISLATTCAGSLPSCCSLSWCVRSLRVSCLMG

IRESfinder result

IRESfinder score

0.309035594

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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