top of page
mmu_circAacs_409
Basic information of circular RNA
PanCircBase ID:
mmu_circAacs_409
mm39_Chr. Position ID:
chr5|125566846|125580694|+
Gene name:
Aacs
Transcript:
ENSMUST00000031445.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,5124,13400,13766
Genomic length:
13848
Exon/intron sizes in nt:
114,98,115,82
Flanking exon/introns:
chr5:125561721-125566846|chr5:125580694-125583212
Splice length:
409
Other database IDs:
circAtlas: hsa-AACS_0010
Human Ortholog IDs:
Mature spliced Sequence:
GGGAAGGCAGAGAGGAGATCGTGAAGGTGACTTTTGAAGAGCTGCGGCAGCAGGTGGCTCTGTTCGCAGCTGCCATGAGGAAGATGGGCGTGAAGAAAGGGGACCGTGTGGTCGGCTATCTACCCAACAGTGCGCATGCGGTGGAGGCCATGCTGGCTGCTGCCAGTATTGGGGCCATTTGGAGTTCTACCTCGCCGGACTTTGGTGTAAATGGTGTCCTGGACCGCTTTTCTCAAATTCAGCCGAAACTTATCTTCTCGGTGGAGGCTGTTGTCTACAATGGCAAGGAGCATGGTCACCTGGAGAAGCTGCAGCGAGTTGTGAAAGGACTGCCTGACCTGCAGCGAGTGGTGCTGATCCCCTATGTCCTCCCAAGGGAGAAGATTGACATTTCCAAGATCCCCAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCCTATGTCCTCCCAAGG
60.03
TCACGCCCATCTTCCTCATG
59.82
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGAAAGGACTGCCTGACC
59.89
CTGCCGCAGCTCTTCAAAAG
60.11
140
PCR template sequence:
TGCAGCGAGTTGTGAAAGGACTGCCTGACCTGCAGCGAGTGGTGCTGATCCCCTATGTCCTCCCAAGGGAGAAGATTGACATTTCCAAGATCCCCAACAGGGGAAGGCAGAGAGGAGATCGTGAAGGTGACTTTTGAAGAGCTGCGGCAGCAGGTGGCTCTGTTCGCAGCTGCCATGAGGAAGATGGGCGTGAAGAAAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-200c-3p(1), mmu-let-7c-5p(1), mmu-miR-29b-3p(1), mmu-miR-29c-3p(1), mmu-miR-98-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AGATCCCCAACAGGGGAAGGCAGAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAACS_409_ORF_1
1.1212
0.401760082
0.849975868
453
ORF sequence:
ATGTCCTCCCAAGGGAGAAGATTGACATTTCCAAGATCCCCAACAGGGGAAGGCAGAGAGGAGATCGTGAAGGTGACTTTTGAAGAGCTGCGGCAGCAGGTGGCTCTGTTCGCAGCTGCCATGAGGAAGATGGGCGTGAAGAAAGGGGACCGTGTGGTCGGCTATCTACCCAACAGTGCGCATGCGGTGGAGGCCATGCTGGCTGCTGCCAGTATTGGGGCCATTTGGAGTTCTACCTCGCCGGACTTTGGTGTAAATGGTGTCCTGGACCGCTTTTCTCAAATTCAGCCGAAACTTATCTTCTCGGTGGAGGCTGTTGTCTACAATGGCAAGGAGCATGGTCACCTGGAGAAGCTGCAGCGAGTTGTGAAAGGACTGCCTGACCTGCAGCGAGTGGTGCTGATCCCCTATGTCCTCCCAAGGGAGAAGATTGACATTTCCAAGATCCCCAAC
Peptide sequence:
MSSQGRRLTFPRSPTGEGREEIVKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHGHLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPN
IRESfinder result
IRESfinder score
0.228691381
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page