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mmu_circ8030462N17Rik_785

Basic information of circular RNA
PanCircBase ID:

mmu_circ8030462N17Rik_785

mm39_Chr. Position ID:

chr18|77761595|77762380|-

Gene name:

8030462N17Rik

Transcript:

ENSMUST00000074653.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

1

Exon/intron indexes:

2

Exon/intron offsets:

0

Genomic length:

785

Exon/intron sizes in nt:

785

Flanking exon/introns:

chr18:77740992-77761595|chr18:77762380-77801420

Splice length:

785

Other database IDs:

circBase: mmu_circ_0007534, CIRCpedia: MMU_CIRCpedia_29751, circAtlas: mmu-8030462N17Rik_0001

circBase: hsa_circ_0007699, circAtlas: hsa-C18orf25_0001, CIRCpedia: HSA_CIRCpedia_23864

Human Ortholog IDs:
Mature spliced Sequence:

GCTGCTTCCCTGTTGCACCTTTGATATCGTTTCCTAGACTCCGTTCCATGATTTAATTTGGAATTCTGAGATGAAGATGGAGGAGGCAGTGGGAAAAGTTGAAGAACTCATTGAGTCTGCAGCCCCACCAAAAGCATCTGAACAAGAGACAGCCAAGGAAGAAGATGGCTCTGTAGAACTGGAATCTCAAGTTCAGAAAGATGGTGTAGCTGACTCTACAGTTCTCTCTTCAATGCCCTGTTTGTTGATGGAACTGAGAAGGGACTCTTCAGAGTCTCAGTTAGCATCCACAGAGAGTGACAAGCCCACAACTGGCCGAGTTTATGAGAGTGACTCCTCTAATCATTGCATGCTTTCCCCTTCCTCTAGTGGCCATTTGGCTGATTCAGATACATTGTCTTCTGTTGAAGAGAATGAACCCTCGCAAGCAGAAACAACCGTGGAAGGAGATACTTCAGGAGTGTCTGGTGCCACTGTTGGACGAAAGTCTCGGCGGTCCCGTTCTGAAAGTGAAACATCTACTATGGCTGCCAAGAAAAACCGGCAATCCAGCGATAAGCAGAATGGCAGAGTCACCAAGGTTAAAGGTCATCGGAGCCAAAAGCACAAGGAGAGGATCAGACTACTGAGGCAGAAACGGGAGGCAGCTGCAAGGAAGAAGTACAACCTGCTGCAGGACAGTAGTACCAGTGACAGTGACCTGACTTGTGACTCAAGCACGAGTTCCTCAGATGATGATGACGAGGTTTCAGGGAGCAGCAAGACAATCACTGCAGAGATACCAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ATGACGAGGTTTCAGGGAGC

59.75

CCACTGCCTCCTCCATCTTC

59.82

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

ATGACGAGGTTTCAGGGAGC

59.75

CTTTTCCCACTGCCTCCTCC

60.32

146

PCR template sequence:

ACCAGTGACAGTGACCTGACTTGTGACTCAAGCACGAGTTCCTCAGATGATGATGACGAGGTTTCAGGGAGCAGCAAGACAATCACTGCAGAGATACCAGGCTGCTTCCCTGTTGCACCTTTGATATCGTTTCCTAGACTCCGTTCCATGATTTAATTTGGAATTCTGAGATGAAGATGGAGGAGGCAGTGGGAAAAGTT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

8

Interacting miRNAs (#binding sites)

mmu-miR-221-3p(1), mmu-miR-18a-5p(1), mmu-miR-495-3p(1), mmu-miR-96-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-1904(1), mmu-miR-3105-3p(1)

siRNAs for circular RNA silencing
Junction sequence

GCAGAGATACCAGGCTGCTTCCCTGT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRC8030462N17RIK_785_ORF_1

0.9893

0.221399768

0.953864152

771

ORF sequence:

ATGAAGATGGAGGAGGCAGTGGGAAAAGTTGAAGAACTCATTGAGTCTGCAGCCCCACCAAAAGCATCTGAACAAGAGACAGCCAAGGAAGAAGATGGCTCTGTAGAACTGGAATCTCAAGTTCAGAAAGATGGTGTAGCTGACTCTACAGTTCTCTCTTCAATGCCCTGTTTGTTGATGGAACTGAGAAGGGACTCTTCAGAGTCTCAGTTAGCATCCACAGAGAGTGACAAGCCCACAACTGGCCGAGTTTATGAGAGTGACTCCTCTAATCATTGCATGCTTTCCCCTTCCTCTAGTGGCCATTTGGCTGATTCAGATACATTGTCTTCTGTTGAAGAGAATGAACCCTCGCAAGCAGAAACAACCGTGGAAGGAGATACTTCAGGAGTGTCTGGTGCCACTGTTGGACGAAAGTCTCGGCGGTCCCGTTCTGAAAGTGAAACATCTACTATGGCTGCCAAGAAAAACCGGCAATCCAGCGATAAGCAGAATGGCAGAGTCACCAAGGTTAAAGGTCATCGGAGCCAAAAGCACAAGGAGAGGATCAGACTACTGAGGCAGAAACGGGAGGCAGCTGCAAGGAAGAAGTACAACCTGCTGCAGGACAGTAGTACCAGTGACAGTGACCTGACTTGTGACTCAAGCACGAGTTCCTCAGATGATGATGACGAGGTTTCAGGGAGCAGCAAGACAATCACTGCAGAGATACCAGGCTGCTTCCCTGTTGCACCTTTGATATCGTTTCCTAGACTCCGTTCCATGATTTAA

Peptide sequence:

MKMEEAVGKVEELIESAAPPKASEQETAKEEDGSVELESQVQKDGVADSTVLSSMPCLLMELRRDSSESQLASTESDKPTTGRVYESDSSNHCMLSPSSSGHLADSDTLSSVEENEPSQAETTVEGDTSGVSGATVGRKSRRSRSESETSTMAAKKNRQSSDKQNGRVTKVKGHRSQKHKERIRLLRQKREAAARKKYNLLQDSSTSDSDLTCDSSTSSSDDDDEVSGSSKTITAEIPGCFPVAPLISFPRLRSMI

IRESfinder result

IRESfinder score

0.52427048

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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