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mmu_circ4932438A13Rik_991

Basic information of circular RNA
PanCircBase ID:

mmu_circ4932438A13Rik_991

mm39_Chr. Position ID:

chr3|36974328|36992723|+

Gene name:

4932438A13Rik

Transcript:

ENSMUST00000057272.15

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

6

Exon/intron indexes:

19,20,21,22,23,24

Exon/intron offsets:

0,1039,8255,10818,16809,18266

Genomic length:

18395

Exon/intron sizes in nt:

1,07,20,92,28,13,81,80,000

Flanking exon/introns:

chr3:36974182-36974328|chr3:36992723-36994901

Splice length:

991

Other database IDs:

​

circAtlas: hsa-KIAA1109_0194

Human Ortholog IDs:
Mature spliced Sequence:

GGCTGGTTGGGTTGAATGCTGGACTGTCCCAAGTGTCATGCTCACCATTGATTATACCTGGCATCCAATTTATCCACAAAAAGCAGATGAACAGCTAAAACAGTCATTGTCAGAAATGGAAGAAACAATGCTATCTGTATTAAGGCCAGCCCAGAAGACATCAGAGAGAGTTGTTTCTTCTCCATCTATGTCACCACGCCCTCCTGTTGATCCCTCAGAGCTTCCACCTGACAAACTTCATGTAGAGATGGAACTCTCCCCAGACTCTCAAATAACTCTCTATGGACCTCTGCTAAATGCCTTCTTATGTATCAAGGAAAACTACTTTGGAGAAGATGACATGTACATGGACTTTGAAGAGGTAATTTCAAGCCCTGTCCTGTCACTGTCAACATCCTCCAGTTCTGGGTGGACCGCTGTTGGAATGGACAATGACAAAAGGGAAAATGAAAGTTCAGCCAAGTCAATCCATCCACTTGCCTTGCGGCCCTGGGATATCACTGTACTTGTTAATTTGTACAAGGTTCATGGGCGCCTTCCTGTGCACGGAACCACCGATGGTCCTGAGTGCCCAACAGCATTTTTGGAGAGACTATGTTTTGAAATGAAAAAGGGATTCAGAGAGACCATGTTGCAACTCGTCTTGTCACCCCTGAATGTGTTTGTCAGTGATAATTATCAGCAGCGACCCCCTGTGGATGAAGTACTCAGGGAAGGTCACATCAATTTGTCAGGTCTCCAGTTGAGAGCACATGCCATGTTCTCAGCTGAAGGTCTTCCCTTAGGAAGTGACTCCTTAGAATATGCATGGCTGATCGATGTGCAGGCTGGAAGCCTTACAGCTAAAGTCACAGCACCACAGCTGGCATGTCTCTTGGAATGGGGGCAGACATTTGTTTTCCATGTGGTATGTCGGGAGTATGAACTGGAAAGACCAAAGTCAGTTATAGTATGCCAACATGGAATTGATCGCCGGTTCTGTGAATCCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TTCCATGTGGTATGTCGGGAG

59.52

TGGTGAGCATGACACTTGGG

60.25

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TTCCATGTGGTATGTCGGGA

58.43

TGGTGAGCATGACACTTGGG

60.25

140

PCR template sequence:

ATTTGTTTTCCATGTGGTATGTCGGGAGTATGAACTGGAAAGACCAAAGTCAGTTATAGTATGCCAACATGGAATTGATCGCCGGTTCTGTGAATCCAAGGGCTGGTTGGGTTGAATGCTGGACTGTCCCAAGTGTCATGCTCACCATTGATTATACCTGGCATCCAATTTATCCACAAAAAGCAGATGAACAGCTAAAA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

11

Interacting miRNAs (#binding sites)

mmu-miR-9-5p(1), mmu-miR-93-5p(1), mmu-miR-181a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-181b-5p(1), mmu-miR-125a-5p(1), mmu-miR-127-5p(1), mmu-miR-670-5p(1), mmu-miR-181d-5p(1), mmu-miR-7a-1-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CTGTGAATCCAAGGGCTGGTTGGGTT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRC4932438A13RIK_991_ORF_1

0.9036

0.052998697

0.981835088

1023

ORF sequence:

ATGGAATTGATCGCCGGTTCTGTGAATCCAAGGGCTGGTTGGGTTGAATGCTGGACTGTCCCAAGTGTCATGCTCACCATTGATTATACCTGGCATCCAATTTATCCACAAAAAGCAGATGAACAGCTAAAACAGTCATTGTCAGAAATGGAAGAAACAATGCTATCTGTATTAAGGCCAGCCCAGAAGACATCAGAGAGAGTTGTTTCTTCTCCATCTATGTCACCACGCCCTCCTGTTGATCCCTCAGAGCTTCCACCTGACAAACTTCATGTAGAGATGGAACTCTCCCCAGACTCTCAAATAACTCTCTATGGACCTCTGCTAAATGCCTTCTTATGTATCAAGGAAAACTACTTTGGAGAAGATGACATGTACATGGACTTTGAAGAGGTAATTTCAAGCCCTGTCCTGTCACTGTCAACATCCTCCAGTTCTGGGTGGACCGCTGTTGGAATGGACAATGACAAAAGGGAAAATGAAAGTTCAGCCAAGTCAATCCATCCACTTGCCTTGCGGCCCTGGGATATCACTGTACTTGTTAATTTGTACAAGGTTCATGGGCGCCTTCCTGTGCACGGAACCACCGATGGTCCTGAGTGCCCAACAGCATTTTTGGAGAGACTATGTTTTGAAATGAAAAAGGGATTCAGAGAGACCATGTTGCAACTCGTCTTGTCACCCCTGAATGTGTTTGTCAGTGATAATTATCAGCAGCGACCCCCTGTGGATGAAGTACTCAGGGAAGGTCACATCAATTTGTCAGGTCTCCAGTTGAGAGCACATGCCATGTTCTCAGCTGAAGGTCTTCCCTTAGGAAGTGACTCCTTAGAATATGCATGGCTGATCGATGTGCAGGCTGGAAGCCTTACAGCTAAAGTCACAGCACCACAGCTGGCATGTCTCTTGGAATGGGGGCAGACATTTGTTTTCCATGTGGTATGTCGGGAGTATGAACTGGAAAGACCAAAGTCAGTTATAGTATGCCAACATGGAATTGATCGCCGGTTCTGTGAATCCAAG

Peptide sequence:

MELIAGSVNPRAGWVECWTVPSVMLTIDYTWHPIYPQKADEQLKQSLSEMEETMLSVLRPAQKTSERVVSSPSMSPRPPVDPSELPPDKLHVEMELSPDSQITLYGPLLNAFLCIKENYFGEDDMYMDFEEVISSPVLSLSTSSSSGWTAVGMDNDKRENESSAKSIHPLALRPWDITVLVNLYKVHGRLPVHGTTDGPECPTAFLERLCFEMKKGFRETMLQLVLSPLNVFVSDNYQQRPPVDEVLREGHINLSGLQLRAHAMFSAEGLPLGSDSLEYAWLIDVQAGSLTAKVTAPQLACLLEWGQTFVFHVVCREYELERPKSVIVCQHGIDRRFCESK

IRESfinder result

IRESfinder score

0.533633698

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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