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mmu_ciPrrc2b_496
Basic information of circular RNA
PanCircBase ID:
mmu_ciPrrc2b_496
mm39_Chr. Position ID:
chr2|32072638|32073134|+
Gene name:
Prrc2b
Transcript:
ENSMUST00000036691.14
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
496
Exon/intron sizes in nt:
496
Flanking exon/introns:
chr2:32072638-32073134
Splice length:
496
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGCCCGGCCGAAGGCCCCTCTTGCGTTCTTTGCTTCCGTCCCTTGTAAGATGGCCAGGCCCGCCAGGCACTTGTGTCTCTAGCCAGTTTGGGCAGGCAGGTGGGGTGACAGCGTTTGCATAGCCTTCCCCCTCTCAGTTTCCTGGGTCTGACTTCCTAGTGGAAGGATTGGCTGCCACCCACAAGGCCTTCCTGGGGGTTCTTGCCGCACAGCCAGGACCATGTCATATTGCAGGGGTGGTCACTGTGGGCTGAGCTTCCTGGGCCTGGCTCCACCGTGGCGTGAGCTCCACAGCGGCCTCCTGCCAAGGTAGAATGTGCAGATCCCAGCAGTCCTTTGTCTCTAGTCTGGGGGAGGAGCTGGCAGAGGCTGGAGTGACTACCAAGCACTTGTCTTGGCTTGACTTGGCTCCTTAGTCTGAGCAGTGGTGTGTATTTTGGCCTCTCTCCTCCTGTGAGGTGTGTGCTCTACCCCATGTTCTTTTCCTTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGGCTTGACTTGGCTCCTT
59.81
TGGCCATCTTACAAGGGACG
59.75
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGGCTTGACTTGGCTCCTT
59.81
GACGGAAGCAAAGAACGCAA
59.69
142
PCR template sequence:
TCTTGGCTTGACTTGGCTCCTTAGTCTGAGCAGTGGTGTGTATTTTGGCCTCTCTCCTCCTGTGAGGTGTGTGCTCTACCCCATGTTCTTTTCCTTGCAGGTAAGGCCCGGCCGAAGGCCCCTCTTGCGTTCTTTGCTTCCGTCCCTTGTAAGATGGCCAGGCCCGCCAGGCACTTGTGTCTCTAGCCAGTTTGGGCAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-184-3p(1), mmu-miR-128-3p(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTTCCTTGCAGGTAAGGCCCGGCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIPRRC2B_496_ORF_1
0.6442
-0.214680584
0.019706252
144
ORF sequence:
ATGTTCTTTTCCTTGCAGGTAAGGCCCGGCCGAAGGCCCCTCTTGCGTTCTTTGCTTCCGTCCCTTGTAAGATGGCCAGGCCCGCCAGGCACTTGTGTCTCTAGCCAGTTTGGGCAGGCAGGTGGGGTGACAGCGTTTGCATAG
Peptide sequence:
MFFSLQVRPGRRPLLRSLLPSLVRWPGPPGTCVSSQFGQAGGVTAFA
IRESfinder result
IRESfinder score
0.68154543
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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