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mmu_ciPpil2_380
Basic information of circular RNA
PanCircBase ID:
mmu_ciPpil2_380
mm39_Chr. Position ID:
chr16|16910168|16910548|-
Gene name:
Ppil2
Transcript:
ENSMUST00000023455.14
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
12
Exon/intron offsets:
0
Genomic length:
380
Exon/intron sizes in nt:
380
Flanking exon/introns:
chr16:16910168-16910548
Splice length:
380
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTGTGATGGCTGTCACCAGGTGGTTCCTGGGGCCACAGGCTAGTCCTGCCTTAAATGTGGAGCTACGGTTGGTTTACTTATAAGAAGCCTTACCTGACTGCCCCTGAGACTCAAAAGGGCCCTGACTTCCTTCCCTGGAGTCCCTTTAGCAACATACCTCCTTAGCTGCTTGGTAAGTGTATCTATGTGCAAATCCTAGGATAGAGCCCTGACTCTGTTTCCCCATGTGTCATATGGCTGTAGGACTCTGTAGCTTAGCTTAACTCTTAGAAGTCAGACTGAACCAAAGCCTACAGAACTTTCTGCTATGATAAAGGTCTGCATGTCTTATGCCTCTGCTATTAACTATGCCTCATACTGCTGGGATATTTTGTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTCTGCATGTCTTATGCCT
59.16
AAACCAACCGTAGCTCCACA
59.53
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTCTGCATGTCTTATGCCT
59.16
AACCAACCGTAGCTCCACAT
59.31
140
PCR template sequence:
GACTGAACCAAAGCCTACAGAACTTTCTGCTATGATAAAGGTCTGCATGTCTTATGCCTCTGCTATTAACTATGCCTCATACTGCTGGGATATTTTGTAGGTGAGTGTGATGGCTGTCACCAGGTGGTTCCTGGGGCCACAGGCTAGTCCTGCCTTAAATGTGGAGCTACGGTTGGTTTACTTATAAGAAGCCTTACCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-202-3p(2), mmu-miR-9-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGATATTTTGTAGGTGAGTGTGATGG
siRNA sets
ACTCACCTACAAAATATCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.590366725
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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