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mmu_ciPolr3h_114
Basic information of circular RNA
PanCircBase ID:
mmu_ciPolr3h_114
mm39_Chr. Position ID:
chr15|81798872|81798986|-
Gene name:
Polr3h
Transcript:
ENSMUST00000230066.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
114
Exon/intron sizes in nt:
114
Flanking exon/introns:
chr15:81773600-81798986
Splice length:
114
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCAGGACAGCCAGCCTGGCTCCTCACTGCTGCAGCTCCTTCATCCTGTTGAGGGCACTGCCTGCACGGAACCACTCGATCTGGGTCTCGTTGAAGGTGTGGTTCAGGAGGATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGTGGTTCAGGAGGATGG
60.04
ACAGGATGAAGGAGCTGCAG
59.75
68
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGTCTCGTTGAAGGTGTG
59.89
ACAGGATGAAGGAGCTGCAG
59.75
82
PCR template sequence:
CTGCCTGCACGGAACCACTCGATCTGGGTCTCGTTGAAGGTGTGGTTCAGGAGGATGGTCAGGACAGCCAGCCTGGCTCCTCACTGCTGCAGCTCCTTCATCCTGTTGAGGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-34b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTCAGGAGGATGGTCAGGACAGCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIPOLR3H_114_ORF_1
1.3131
0.102133139
0.175862493
117
ORF sequence:
ATGGTCAGGACAGCCAGCCTGGCTCCTCACTGCTGCAGCTCCTTCATCCTGTTGAGGGCACTGCCTGCACGGAACCACTCGATCTGGGTCTCGTTGAAGGTGTGGTTCAGGAGGATG
Peptide sequence:
MVRTASLAPHCCSSFILLRALPARNHSIWVSLKVWFRRM
IRESfinder result
IRESfinder score
0.614001553
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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