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mmu_ciNbr1_217

Basic information of circular RNA
PanCircBase ID:

mmu_ciNbr1_217

mm39_Chr. Position ID:

chr11|101463398|101463615|+

Gene name:

Nbr1

Transcript:

ENSMUST00000103099.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

17

Exon/intron offsets:

0

Genomic length:

217

Exon/intron sizes in nt:

217

Flanking exon/introns:

chr11:101463398-101463633

Splice length:

217

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGGGGTTAAATATCTGTAAAGCACGTGCCTTCCCTTTCCATCCACCTCTCCTCACACGTCCATTCCTCATTACCTCTACCTCAGCAAATCGATGTCCCATGGCATCCCTCGCTCTCTACGTCTCATGACAGCAGGGGCAGCTCTTCCCCCACCCAGGCTTAGGATGCACTGACTGGTGAGAGCTCTCAGCTCTTCAGACACTAAGTACAGGCAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TACGTCTCATGACAGCAGGG

59.18

TGGATGGAAAGGGAAGGCAC

59.96

145

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TACGTCTCATGACAGCAGGG

59.18

GTGGATGGAAAGGGAAGGCA

59.96

146

PCR template sequence:

TCTACGTCTCATGACAGCAGGGGCAGCTCTTCCCCCACCCAGGCTTAGGATGCACTGACTGGTGAGAGCTCTCAGCTCTTCAGACACTAAGTACAGGCAGGTAAGGGGTTAAATATCTGTAAAGCACGTGCCTTCCCTTTCCATCCACCTCTCCTCACACGTCCATTCCTCATTACCTCTACCTCAGCAAATCGATGTCC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

8

Interacting miRNAs (#binding sites)

mmu-miR-124-3p(1), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-148b-3p(1), mmu-let-7d-5p(1), mmu-miR-370-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TAAGTACAGGCAGGTAAGGGGTTAAA

siRNA sets

TTTAACCCCTTACCTGCCT,TTAACCCCTTACCTGCCTG,TAACCCCTTACCTGCCTGT

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.572978395

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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