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mmu_ciNaa60_321
Basic information of circular RNA
PanCircBase ID:
mmu_ciNaa60_321
mm39_Chr. Position ID:
chr16|3717832|3718153|+
Gene name:
Naa60
Transcript:
ENSMUST00000151988.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
321
Exon/intron sizes in nt:
321
Flanking exon/introns:
chr16:3717832-3718506
Splice length:
321
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGAAGCAGAGAGTTCATGGTTTAAACCACCCCTCCCCTAGCCAGTTAACACCCTTGTAACCCAAACTAGTCACTTCTTCTGAGGTCTTCATCTTAGAGGACCCTTAGTCTCTTCTTCCTTATCCCTATTTGGGGACAAATCCTGTCTTCCTGTCCTCCCAGTGTGTCTCCCTAGGACAATCTCAGGGAAGAACAGACCTCCCTTACCTCTCCTGTTGCCAGACATAGTCGTGATCTATTGGCAAGATATTCTCAGTTCAGAATAAGAAACATAAATGAAGTCATGGCACTCTGCAGCATTTATCAGACCCTTTGTTAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCGTGATCTATTGGCAAGA
56.83
GGGTGTTAACTGGCTAGGGG
59.74
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCGTGATCTATTGGCAAGA
56.83
TTAACTGGCTAGGGGAGGGG
60.33
144
PCR template sequence:
CAGACATAGTCGTGATCTATTGGCAAGATATTCTCAGTTCAGAATAAGAAACATAAATGAAGTCATGGCACTCTGCAGCATTTATCAGACCCTTTGTTATGTAAGAAGCAGAGAGTTCATGGTTTAAACCACCCCTCCCCTAGCCAGTTAACACCCTTGTAACCCAAACTAGTCACTTCTTCTGAGGTCTTCATCTTAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACCCTTTGTTATGTAAGAAGCAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.87608623
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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