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mmu_ciMetap2_430

Basic information of circular RNA
PanCircBase ID:

mmu_ciMetap2_430

mm39_Chr. Position ID:

chr10|93715504|93715934|-

Gene name:

Metap2

Transcript:

ENSMUST00000181091.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

3

Exon/intron offsets:

0

Genomic length:

430

Exon/intron sizes in nt:

430

Flanking exon/introns:

chr10:93715435-93715934

Splice length:

430

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTTAGTGTCTTACGAGTCTTATTAGATTGACAGTGACAGTGGCCCTGGGTCTGAGGAAGACTTAGAACTTGCTCTAGACCATAATCATGTCAGAGAAACAAACAGGAGCTTGAATTAGCAGTCTTGTGGGCATTGTCTTTAGCTGTCAGCCTCAGGATTGTTAGTAGGATTGGTTGCCTTTGCAGACTTAGTATTTCTACAAAGGACAGTAGAAGCAATTCTTCTATTAGAGCAGCAAGAAGAACATAATTTCTGACTGTCTACTAGCTCTGAAATGACTAGATTTTGGGTAAATCCTTAACGAGAGGTCCCATAATTTAAAGTATGAATATGGTAAGGTTTTATTAATAACTTGTATATTGTACTGTTCAAATATGGTTCTGTTTTATTGTTTTTGTAGATAGTATAACTGAATATTTGTATCTCACAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGTACTGTTCAAATATGGTTCTGT

56.3

AGCAAGTTCTAAGTCTTCCTCAGA

59.16

143

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGTACTGTTCAAATATGGTTCTGT

56.3

AGAGCAAGTTCTAAGTCTTCCTCA

59.16

145

PCR template sequence:

ATGGTAAGGTTTTATTAATAACTTGTATATTGTACTGTTCAAATATGGTTCTGTTTTATTGTTTTTGTAGATAGTATAACTGAATATTTGTATCTCACAGGTTAGTGTCTTACGAGTCTTATTAGATTGACAGTGACAGTGGCCCTGGGTCTGAGGAAGACTTAGAACTTGCTCTAGACCATAATCATGTCAGAGAAACA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

3

Interacting miRNAs (#binding sites)

mmu-miR-101a-3p(1), mmu-miR-499-5p(1), mmu-miR-5101(1)

siRNAs for circular RNA silencing
Junction sequence

TTGTATCTCACAGGTTAGTGTCTTAC

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.877604369

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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