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mmu_ciMan2a2_986
Basic information of circular RNA
PanCircBase ID:
mmu_ciMan2a2_986
mm39_Chr. Position ID:
chr7|80019493|80020479|-
Gene name:
Man2a2
Transcript:
ENSMUST00000098346.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
986
Exon/intron sizes in nt:
986
Flanking exon/introns:
chr7:80019493-80020479
Splice length:
986
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCAAGTGGGGACTCGCGGGCGGTGGCGGCAGGCACTGTGGGTCGTCTCGAGTTGCCCCGTTTCGGAGCCGCTGTGCCTGGCTTCCGCTACCCTGGGGACTTTATCAGTCTCGCCGCTAGCTTCCTAAGAGTCCCTTTAAAGCACTCTGGAAGCCAGGACTGGAAGTTTTGCACAGCAGGAATCAGTACTGATGGGGACAATCCTAGAACTGGGTTGCGTTAGAAACATGGGCATTTCTTTTTCTCTTAAAAGAAATCCATTCTGTATTCTCTGAGATAGGATCTGGGCCAGGCACTGTTTCTGCTGAGTTCCAGTCAGCCCCAGGAGGTCACAGGGGAGGCAGGGTGTGAGTGTGTGTGTGTGGGGGGGTTGTCTGTCTCTCCAGCTGGTGATTGTACCCCTAAATCAAACAAAATAAAACCTCTTGTATTTATGTATACATCTGAAGGGAGGAAACACGTGGCTTGGGAGAGCCTTCTCAGAGTCCTGAGCAAGCTGTTTGCAATCAGTGGTTGTGTAATGAAGTGTGGGTCAGAGTTGGGGCTCTGTTATCCCTGTTTGTTTGTTTGTAATGTGTTACATTTATTTATGTGCATGTACGTCACAGAAAACATGCGGCAATCAGAGGACAATTTGTGGTAGTTGGTTTTCACCTTCCACCATATGAATCAAATGAATCGCTTTGGTTGTCAGGCTTGCCTGCAAGTGCCTCTTTACCTACTGCACCCCCACACTCCACATCCTGGTTTCTATAGCTTGGGCTGAGGGGAATCAGAACTCCTCTCCTACAGGACCAGCCCCAAATATATGTGCTACTTGTGTGTTGGGAGTCATAACCAAGATTTGGTACTTGAAAGCTTACTTGCTTTTTTACCCAGAGTCAAGCAAGTAGGTTGGTTCTCCTTGGGAGTCCACAGAGCGTTTGTTAGGAACACCAACAGAAGGGTTTTGCATGCCAGAACTACAGGTGGTGTCTCTCCTGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTTGGGAGTCCACAGAG
59.1
CAACTCGAGACGACCCACAG
60.39
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTTGGGAGTCCACAGAGC
61.19
CAACTCGAGACGACCCACAG
60.39
140
PCR template sequence:
AAGTAGGTTGGTTCTCCTTGGGAGTCCACAGAGCGTTTGTTAGGAACACCAACAGAAGGGTTTTGCATGCCAGAACTACAGGTGGTGTCTCTCCTGCCAGGCAAGTGGGGACTCGCGGGCGGTGGCGGCAGGCACTGTGGGTCGTCTCGAGTTGCCCCGTTTCGGAGCCGCTGTGCCTGGCTTCCGCTACCCTGGGGACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(2), mmu-miR-495-3p(2), mmu-miR-19a-3p(1), mmu-miR-185-5p(1), mmu-miR-411-3p(1), mmu-miR-370-3p(1), mmu-miR-466d-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-342-3p(1), mmu-miR-5101(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTCTCCTGCCAGGCAAGTGGGGACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIMAN2A2_986_ORF_1
0.4657
-0.207457144
0.04465111
306
ORF sequence:
ATGTGCTACTTGTGTGTTGGGAGTCATAACCAAGATTTGGTACTTGAAAGCTTACTTGCTTTTTTACCCAGAGTCAAGCAAGTAGGTTGGTTCTCCTTGGGAGTCCACAGAGCGTTTGTTAGGAACACCAACAGAAGGGTTTTGCATGCCAGAACTACAGGTGGTGTCTCTCCTGCCAGGCAAGTGGGGACTCGCGGGCGGTGGCGGCAGGCACTGTGGGTCGTCTCGAGTTGCCCCGTTTCGGAGCCGCTGTGCCTGGCTTCCGCTACCCTGGGGACTTTATCAGTCTCGCCGCTAGCTTCCTAA
Peptide sequence:
MCYLCVGSHNQDLVLESLLAFLPRVKQVGWFSLGVHRAFVRNTNRRVLHARTTGGVSPARQVGTRGRWRQALWVVSSCPVSEPLCLASATLGTLSVSPLAS
IRESfinder result
IRESfinder score
0.734791172
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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