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mmu_ciKif1a_1069
Basic information of circular RNA
PanCircBase ID:
mmu_ciKif1a_1069
mm39_Chr. Position ID:
chr1|93002803|93003872|-
Gene name:
Kif1a
Transcript:
ENSMUST00000171796.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
1069
Exon/intron sizes in nt:
1069
Flanking exon/introns:
chr1:93002789-93003872
Splice length:
1069
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTGGGGTGACTTGTTCTCCTTAGCCACACTCTCCCCTCTTGCCCTGTCCTTATAGCACTACTAGCCTCACCTCATGATAAGTCAGTGTGGTGGCTCCCAAGAGGATAAAGGACCTCATGTGGCAGGCCACTGAGGACACATATCAGCTTGAGAGAGGCCTTGGTTTCCTCCTGCATGAGGGCTGCTCCTGGGCTGAGCATATGGGCTGTGTGGCTCTGTTACCTCAGCAACAGGACTCTGACAGCAATATCAATAGCTCCGAGGTAGTCTTTGTGCCCAAGGGCAGCTTTTCTGTCAAGCCTAGGTCTAGAAACTGTGAGGGTCATCATGGTGACCATAAGCAATCTACCCCCTTTTTTTAGCCTGGGACACTGGTCGCCATGATGGTCTTACTGACACATATTGGTAGCCCCAGATCGCTCAGGTGGCTGCCTTCAGCTCCTGAGGGTGTGTGCATGTGTGTCCCAAACTACCCCTAACAGCCCCTCAGACTTGTTTATAATAAAGCTCCGTAGTACATGTAAGACTGTGACCTTTGCATACCAGAGATAGGTTCTGAGCTGTTGCCATGAACCCCAACTTGGGAGATTCTATGAGGTTCTTGGCAGGTTGGGGCAGTGTTGAGACCAATCCCCCCACCCATCTCAGGCAGCCTCGGACTTTTTACCCTGAAGAGCCACTTATATGGTCCCTATTACCTCCTGTGGGCTTTGGCTACTGGGGACATGGTATGCTCTTTGGGTTCCCCAGAGTCACTGGAGGAACATTGAGATGGGTGATGGACACTTTCCCCTTCCAAGGGGTGTCCCCACATCAACGCCTGTGAGGATTTCTGTCCAGTTGGGTGATATTGATTCTCAGTAGCAGGAGGCATCTGAGACACCATTTCCCCCAATATGCATGACAGCATAATTGACAAACAGGTGGGAGTTGGGCTTGAGTTCTGGCAGGCAGTGTGGTCACACCCAGGTTGGAGAGTGAGTTCCAGTGACCTGGGTGAGCACCCTGGTACCTCTGGTAATGCTCAGACCTGACCCGGGGTAAGGGGCAGATAACCTCAAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGGTTGGAGAGTGAGTTCC
60
AGAGGGGAGAGTGTGGCTAA
59.59
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGGTTGGAGAGTGAGTTCC
60
AAGAGGGGAGAGTGTGGCTA
59.59
144
PCR template sequence:
CCAGGTTGGAGAGTGAGTTCCAGTGACCTGGGTGAGCACCCTGGTACCTCTGGTAATGCTCAGACCTGACCCGGGGTAAGGGGCAGATAACCTCAAGCAGGTATGTGGGGTGACTTGTTCTCCTTAGCCACACTCTCCCCTCTTGCCCTGTCCTTATAGCACTACTAGCCTCACCTCATGATAAGTCAGTGTGGTGGCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(2), mmu-let-7d-5p(2), mmu-miR-324-3p(2), mmu-miR-137-3p(1), mmu-miR-200a-3p(1), mmu-miR-27b-3p(1), mmu-miR-199a-5p(1), mmu-let-7c-5p(1), mmu-miR-194-5p(1), mmu-miR-1198-5p(1), mmu-miR-3087-3p(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAACCTCAAGCAGGTATGTGGGGTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.718937042
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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