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mmu_ciKif12_399
Basic information of circular RNA
PanCircBase ID:
mmu_ciKif12_399
mm39_Chr. Position ID:
chr4|63084771|63085170|-
Gene name:
Kif12
Transcript:
ENSMUST00000156618.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
16
Exon/intron offsets:
0
Genomic length:
399
Exon/intron sizes in nt:
399
Flanking exon/introns:
chr4:63084747-63085170
Splice length:
399
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGAAGGTACTGAAGACACCGATCAGCTCAGCAGTCCTGGCCATCCCAGGGCTCTGGATGGGGGTGCAGGAGTGGAGGCAGATGCTGGGTCACTCTCTCTGCTCAGAGCATGGAATGTCTGCGTTCACACATGTCCATTCACTTCTGCATTCGTTTCCTCATCCGTTCACTCATTCCTTCTGGAAGCTTCCCTAAGTCGGCCTAAGCTTTGGGCCCCACTCTGTTTTGACCCCCGCCTTCAAGGAACTCCCACTCCCTGGGGGAGACATTCCAAGAACCACCTGTAGTCCATGGAGGAAATGAGACACCCAGCTCTGTCCTTGGCATCTTGTGCAACAGGCATGGCTAGACTCAGCATGGAGGAAGTGGTACCCAGACATGAAAGGCTGAGGCCACC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCATGGAGGAAGTGGTACCC
60.11
GAGAGTGACCCAGCATCTGC
60.46
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCATGGCTAGACTCAGCAT
59.89
GAGAGTGACCCAGCATCTGC
60.46
158
PCR template sequence:
AAATGAGACACCCAGCTCTGTCCTTGGCATCTTGTGCAACAGGCATGGCTAGACTCAGCATGGAGGAAGTGGTACCCAGACATGAAAGGCTGAGGCCACCGTAGGAAGGTACTGAAGACACCGATCAGCTCAGCAGTCCTGGCCATCCCAGGGCTCTGGATGGGGGTGCAGGAGTGGAGGCAGATGCTGGGTCACTCTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCTGAGGCCACCGTAGGAAGGTACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIKIF12_399_ORF_3
0.9229
0.020712629
0.065387105
111
ORF sequence:
ATGGAGGAAATGAGACACCCAGCTCTGTCCTTGGCATCTTGTGCAACAGGCATGGCTAGACTCAGCATGGAGGAAGTGGTACCCAGACATGAAAGGCTGAGGCCACCGTAG
Peptide sequence:
MEEMRHPALSLASCATGMARLSMEEVVPRHERLRPP
IRESfinder result
IRESfinder score
0.589976409
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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