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mmu_ciIns2_491
Basic information of circular RNA
PanCircBase ID:
mmu_ciIns2_491
mm39_Chr. Position ID:
chr7|142232592|142233083|-
Gene name:
Ins2
Transcript:
ENSMUST00000125933.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
491
Exon/intron sizes in nt:
491
Flanking exon/introns:
chr7:142232470-142233083
Splice length:
491
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTTCTGCCACTGAATTCTGTCCCCAGTGCTAACTACCCTGGTTTTCTTCACACTTGGGACATTGTAAATTGTGTCCTAGGTGTGGAGGGTCTCGGGATAACCAGGGAGTGGGGACACGTTTCTGGGGGAAGCTAGACATATGTAAACATGGCAGCTGCCAGGAATGAGTAAGAATCCTGCCTTAAGGGGTCCTTGGTGGTAGTAACTTGGGACATGTGACTAGATCCCAGGATAGGTACCTATTTAGGGCCCTCATAGAGCACTGCACTGACTGAAGATGAGTAGGCTTTAGAGGCCCATGTGTCCATCCATGACCAGTGACTTGTCCCACAGGCATGCAACCCCTGCCACCTGCAGGGGTTAAGGGGCGAGAAAACCTGGGGTAGTAGGAGGTTGCTCAGCTACTCCTGACTGGATTTTCCTATGTGTCTTTGCTTCTGTGCTGCTGATGCCCTGGCCTGCTCTGACACAACCTCCCTGGCAGTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGGTTGCTCAGCTACTCC
59.82
AGGGTAGTTAGCACTGGGGA
59.58
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCTTTGCTTCTGTGCTGC
59.34
AGACCCTCCACACCTAGGAC
59.96
157
PCR template sequence:
AGGAGGTTGCTCAGCTACTCCTGACTGGATTTTCCTATGTGTCTTTGCTTCTGTGCTGCTGATGCCCTGGCCTGCTCTGACACAACCTCCCTGGCAGTGGGTGAGTTCTGCCACTGAATTCTGTCCCCAGTGCTAACTACCCTGGTTTTCTTCACACTTGGGACATTGTAAATTGTGTCCTAGGTGTGGAGGGTCTCGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-15b-5p(1), mmu-miR-22-3p(1), mmu-miR-224-5p(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCCTGGCAGTGGGTGAGTTCTGCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIINS2_491_ORF_1
0.7665
-0.015202069
0.034948826
81
ORF sequence:
ATGTGTCTTTGCTTCTGTGCTGCTGATGCCCTGGCCTGCTCTGACACAACCTCCCTGGCAGTGGGTGAGTTCTGCCACTGA
Peptide sequence:
MCLCFCAADALACSDTTSLAVGEFCH
IRESfinder result
IRESfinder score
0.643385295
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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