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mmu_ciIns2_415

Basic information of circular RNA
PanCircBase ID:

mmu_ciIns2_415

mm39_Chr. Position ID:

chr7|142232668|142233083|-

Gene name:

Ins2

Transcript:

ENSMUST00000105931.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

2

Exon/intron offsets:

0

Genomic length:

415

Exon/intron sizes in nt:

415

Flanking exon/introns:

chr7:142232639-142233083

Splice length:

415

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTGAGTTCTGCCACTGAATTCTGTCCCCAGTGCTAACTACCCTGGTTTTCTTCACACTTGGGACATTGTAAATTGTGTCCTAGGTGTGGAGGGTCTCGGGATAACCAGGGAGTGGGGACACGTTTCTGGGGGAAGCTAGACATATGTAAACATGGCAGCTGCCAGGAATGAGTAAGAATCCTGCCTTAAGGGGTCCTTGGTGGTAGTAACTTGGGACATGTGACTAGATCCCAGGATAGGTACCTATTTAGGGCCCTCATAGAGCACTGCACTGACTGAAGATGAGTAGGCTTTAGAGGCCCATGTGTCCATCCATGACCAGTGACTTGTCCCACAGGCATGCAACCCCTGCCACCTGCAGGGGTTAAGGGGCGAGAAAACCTGGGGTAGTAGGAGGTTGCTCAGCTACTCCTGA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GGGGTTAAGGGGCGAGAAAA

59.96

AGACCCTCCACACCTAGGAC

59.96

151

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGGGTTAAGGGGCGAGAAAA

59.96

GACCCTCCACACCTAGGACA

60.25

150

PCR template sequence:

TGACCAGTGACTTGTCCCACAGGCATGCAACCCCTGCCACCTGCAGGGGTTAAGGGGCGAGAAAACCTGGGGTAGTAGGAGGTTGCTCAGCTACTCCTGAGTGAGTTCTGCCACTGAATTCTGTCCCCAGTGCTAACTACCCTGGTTTTCTTCACACTTGGGACATTGTAAATTGTGTCCTAGGTGTGGAGGGTCTCGGG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

3

Interacting miRNAs (#binding sites)

mmu-miR-22-3p(1), mmu-miR-224-5p(1), mmu-miR-344-3p(1)

siRNAs for circular RNA silencing
Junction sequence

CAGCTACTCCTGAGTGAGTTCTGCCA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.62230817

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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