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mmu_ciGstz1_292
Basic information of circular RNA
PanCircBase ID:
mmu_ciGstz1_292
mm39_Chr. Position ID:
chr12|87206494|87206786|+
Gene name:
Gstz1
Transcript:
ENSMUST00000222885.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
292
Exon/intron sizes in nt:
292
Flanking exon/introns:
chr12:87206494-87206823
Splice length:
292
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTAGAGGACTTGGGGGTGGCAGAGTTCTGAATGAGGGGCCCTGACATCTCTTCCTAACTAACCTACAAGTGACCAAGCATAATAGGGATCATTAAGTGTATGGTAGAGGGAAGGAGAGAGGAAGGTTAAGCATGTGGGGACCCAAGTCCAGCAAGGTCCCCAAAGGACCTTTATAGTGCTCCAGTTGAGGAAGGTGTAGGGGAGAGTTGTGGTACCATTCATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGTTTGCTGCCTCTCCCCTCTGGTCCAGGGATTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGGAGAGTTGTGGTACCA
59.81
GATGTCAGGGCCCCTCATTC
60.18
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGTAGGGGAGAGTTGTGG
59.39
GGGCCCCTCATTCAGAACTC
60.11
141
PCR template sequence:
GGAAGGTGTAGGGGAGAGTTGTGGTACCATTCATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGTTTGCTGCCTCTCCCCTCTGGTCCAGGGATTCGTGAGTAGAGGACTTGGGGGTGGCAGAGTTCTGAATGAGGGGCCCTGACATCTCTTCCTAACTAACCTACAAGTGACCAAGCATAATAGGGATCATTAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTCCAGGGATTCGTGAGTAGAGGAC
siRNA sets
TACTCACGAATCCCTGGAC, TCTACTCACGAATCCCTGG, TCCTCTACTCACGAATCCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.660098965
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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