top of page
mmu_ciGpx8_445
Basic information of circular RNA
PanCircBase ID:
mmu_ciGpx8_445
mm39_Chr. Position ID:
chr13|113182260|113182705|-
Gene name:
Gpx8
Transcript:
ENSMUST00000231962.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
445
Exon/intron sizes in nt:
445
Flanking exon/introns:
chr13:113179833-113182705
Splice length:
445
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTCTTCCGGCTTTTATTTCTGTTGTTCCTTGGCCCTGACACTTATTCTCAGCATAGAGTGGGGTTCGGCACAGCCATAAGTTGGAATGTAGTCTTCAAAGTAATATCGTTAGTGGTCCTTTTAGTTATCAGAATTCTAAGAGCTTTAAACTTCCACTCCACAAAGCAACTGTTTTTCTCGAAAGCTGTCTTCTCTTTTGTGAGGAAACGATGCTGTATTATCTAGGAGATGCTTTGAGACCCGCTACTAAGAGTGTTCTTTACATAACCTAACTAAAACTTTGTCACAGTCTTCGACCATTCATTTGGGTTATGGTTTTCAATAAAGTTGGATCTGTCTAAAAATATGGAATCTATCAGACTTCGTAGTCTGCCAAAATGGCTTAGGATTTTAGCTGTATGAAGATTTTTTTTTTAATCTTTTGCTTCTCTTTCCGGTGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTTCGTAGTCTGCCAAAATGG
58.93
CCGAACCCCACTCTATGCTG
60.18
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACTTCGTAGTCTGCCAAAATGG
59.87
CCGAACCCCACTCTATGCTG
60.18
155
PCR template sequence:
AAATATGGAATCTATCAGACTTCGTAGTCTGCCAAAATGGCTTAGGATTTTAGCTGTATGAAGATTTTTTTTTTAATCTTTTGCTTCTCTTTCCGGTGGGGTAAGTCTTCCGGCTTTTATTTCTGTTGTTCCTTGGCCCTGACACTTATTCTCAGCATAGAGTGGGGTTCGGCACAGCCATAAGTTGGAATGTAGTCTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-127-5p(1), mmu-miR-325-3p(1), mmu-miR-369-3p(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTTTCCGGTGGGGTAAGTCTTCCGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIGPX8_445_ORF_1
0.7593
-0.362259889
0.011847137
126
ORF sequence:
ATGAAGATTTTTTTTTTAATCTTTTGCTTCTCTTTCCGGTGGGGTAAGTCTTCCGGCTTTTATTTCTGTTGTTCCTTGGCCCTGACACTTATTCTCAGCATAGAGTGGGGTTCGGCACAGCCATAA
Peptide sequence:
MKIFFLIFCFSFRWGKSSGFYFCCSLALTLILSIEWGSAQP
IRESfinder result
IRESfinder score
0.878298119
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page