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mmu_ciGolga4_1264
Basic information of circular RNA
PanCircBase ID:
mmu_ciGolga4_1264
mm39_Chr. Position ID:
chr9|118364542|118365806|+
Gene name:
Golga4
Transcript:
ENSMUST00000084820.6
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
8
Exon/intron offsets:
0
Genomic length:
1264
Exon/intron sizes in nt:
1264
Flanking exon/introns:
chr9:118364542-118365826
Splice length:
1264
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGCTCCTGTGTCAGAGCATAGTTCAAACATGAGATGACAGTGTCGAGCATTCAGGATTCAGGCTGAAAGAACAGACCCTAGCATTTCTCCACCTATTCAATTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTTTAAATTGACTTTTTAGCTAGGTAGTGTTAGTGCTTGCCTTTAATCCCAGCACTTGGGAGGCAGAGACAGGAGAATCTCTTGAGTTCGATGCCATCCTGTTCTGCACTGTGAGTTCTAGGACAGCCAGAGCTATACAGAGAAACCTTGTCTTGGAAAAACCAACCAGCAAATAAAAAACCAACCGATTTATTTATTTATTTATTTATTTATATTCAGAAAAAGGGCAATTGATTTGAGACTAAAAAATTGCATAAACTTTAAGGAGAGCAAACATTTTAAAATAATTGAAGTATATAAATTATGTCATACTATTTTGGTTTTTTTGAGACAGGGTCTCATTATATAACCTGTATTGCTCAAGTTGTGATTTTTTTTTTCAGCCTTAGCCTTCCAAGACCAGGCTTACAGGTTTGCATCCACATCTGCCTTATGTCTTCGTTGTCTTACCAAGAGACTCACAGTAGACTTTGGTTCCGTTAGAGGCAAAATAAGTTGTGAGCAGCTGGCCATCTGAGTGATGAGCCTACAAGGGACTGCAGTTCCTGTAGGTATGAGGACGGACTCAAACGTAGGCGGTAAAGCCTATAGTAACTAGAACTCCTTTCAGGAGGAGAGTAGGACTAGAAAGAGCACTCAGTACTTTTGGGATGGAACTTATCAGGGCTTGGGGTGGCCCCCCTCCCCTCAGTTTTGACTTTTAAGTAGGCTAGATCCAGTTAATGCCAGTGTCTGTGCCATTCCAGGGTAAGGAGTTCATTTTATACTTGATTCTTCATCTAAGCATCACACAGTAACCAAGAGTATTTTGAAGAATGCCACTTGCATACGGGGGTGTTCCTTCTCAGTGTAGAAGAAGGCTGTGCAGGGACTGGGCTGTAGCTCCGTGGTAGAGTGTGTGTTGAGCTTGCAGAAGACTCAGCAAAGCTCTCCAGATCCCTGCCCTCCTCATCCCCTAAAAAGATGTCACTGTTGGCAGAGTTCCTTGTAACCCTTAGGCTTTTTAAGCTCATCTCCTCAGTGTTGGTGTGCATGTGGAGGTCAGAGGACCTCGCCAGCACGTGGGTCCTTCGCACACTAGGCCATCTCATGTCTCCTCACTTGTGTGTTTAGTGACTGCCCTGCTGATGCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCACACTAGGCCATCTCAT
59.89
GGGTCTGTTCTTTCAGCCTGA
59.93
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCACACTAGGCCATCTCAT
59.89
AGGGTCTGTTCTTTCAGCCTG
59.93
143
PCR template sequence:
ATGTGGAGGTCAGAGGACCTCGCCAGCACGTGGGTCCTTCGCACACTAGGCCATCTCATGTCTCCTCACTTGTGTGTTTAGTGACTGCCCTGCTGATGCTGTAAGCTCCTGTGTCAGAGCATAGTTCAAACATGAGATGACAGTGTCGAGCATTCAGGATTCAGGCTGAAAGAACAGACCCTAGCATTTCTCCACCTATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-320-3p(1), mmu-miR-339-5p(1), mmu-miR-10b-5p(1), mmu-miR-495-3p(1), mmu-miR-214-3p(1), mmu-miR-191-5p(1), mmu-miR-183-5p(1), mmu-miR-690(1), mmu-miR-207(1), mmu-miR-300-3p(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCTGCTGATGCTGTAAGCTCCTGTG
siRNA sets
AGCTTACAGCATCAGCAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.801891388
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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