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mmu_ciGm15943_77

Basic information of circular RNA
PanCircBase ID:

mmu_ciGm15943_77

mm39_Chr. Position ID:

chr15|58078077|58078154|+

Gene name:

Gm15943

Transcript:

ENSMUST00000147638.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

1

Exon/intron offsets:

0

Genomic length:

77

Exon/intron sizes in nt:

77

Flanking exon/introns:

chr15:58078077-58183835

Splice length:

77

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTATGCGGCGCGTTGGGAAGATGCTGGGGAACGACGCAGGCGGCGACCGGGACAAGAGTGGGTCAACTAGTGTGGAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CGGGACAAGAGTGGGTCAAC

60.6

TTCCCCAGCATCTTCCCAAC

59.96

61

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CGGGACAAGAGTGGGTCAAC

60.6

GTTCCCCAGCATCTTCCCAA

59.96

62

PCR template sequence:

GCGGCGACCGGGACAAGAGTGGGTCAACTAGTGTGGAGGTATGCGGCGCGTTGGGAAGATGCTGGGGAACGACGCA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

NA

Interacting miRNAs (#binding sites)

NA

siRNAs for circular RNA silencing
Junction sequence

AACTAGTGTGGAGGTATGCGGCGCGT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIGM15943_77_ORF_1

0.7757

0.172490398

0.117478122

150

ORF sequence:

ATGCGGCGCGTTGGGAAGATGCTGGGGAACGACGCAGGCGGCGACCGGGACAAGAGTGGGTCAACTAGTGTGGAGGTATGCGGCGCGTTGGGAAGATGCTGGGGAACGACGCAGGCGGCGACCGGGACAAGAGTGGGTCAACTAGTGTGG

Peptide sequence:

MRRVGKMLGNDAGGDRDKSGSTSVEVCGALGRCWGTTQAATGTRVGQLVW

IRESfinder result

IRESfinder score

0.025184521

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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