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mmu_ciGfus_427

Basic information of circular RNA
PanCircBase ID:

mmu_ciGfus_427

mm39_Chr. Position ID:

chr15|75799850|75800277|-

Gene name:

Gfus

Transcript:

ENSMUST00000230610.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

2

Exon/intron offsets:

0

Genomic length:

427

Exon/intron sizes in nt:

427

Flanking exon/introns:

chr15:75799047-75800277

Splice length:

427

Other database IDs:

​

​

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTTGGAGTGCTCCAGGGGCAACAAGCATTGGGAGCCAGGAGCTTAGGTTCTTGAAACTTCCACAGGTTTACCCCCAAGAAGTCCCCGAGGGCTGGTTTTGTACAGCCCTCAGGACATTTGGCCAAGTGCCGACCTGCAGATTGAGAAGCTGAAAGAAACCTGCACACCACACTATGTATCTGGGACTGAGCTTGCTTGGCTGCTAGATTGTCTGTTTGTCTGTCTGTCTATATCTATTTACGTAATGTGCATTGGTGTTTTGCTTGTATGTATGTCTGTGTGAGGATGTCTAGTCTCTTGGAACTTGAGGTACAGACAGTTATGAGCTAGCATGTGGGTGCTGGGATTTGATCCTTTGGAAGAGCAGCCAGTGCTCTTAACTGCTGAGCCATCTCTCTGACCCCCAAATTTTTCTTTCTTTCT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TCCTTTGGAAGAGCAGCCAG

59.96

TGGGGGTAAACCTGTGGAAG

59.23

152

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCCTTTGGAAGAGCAGCCAG

59.96

CTTGGGGGTAAACCTGTGGA

59.23

154

PCR template sequence:

GAGCTAGCATGTGGGTGCTGGGATTTGATCCTTTGGAAGAGCAGCCAGTGCTCTTAACTGCTGAGCCATCTCTCTGACCCCCAAATTTTTCTTTCTTTCTGTAAGTTGGAGTGCTCCAGGGGCAACAAGCATTGGGAGCCAGGAGCTTAGGTTCTTGAAACTTCCACAGGTTTACCCCCAAGAAGTCCCCGAGGGCTGGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

7

Interacting miRNAs (#binding sites)

mmu-miR-150-5p(1), mmu-miR-149-5p(1), mmu-miR-466d-3p(1), mmu-miR-342-3p(1), mmu-miR-484(1), mmu-miR-466b-3p(1), mmu-miR-542-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TTTCTTTCTTTCTGTAAGTTGGAGTG

siRNA sets

CAACTTACAGAAAGAAAGA, AACTTACAGAAAGAAAGAA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.812771265

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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