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mmu_ciG6pc2_629
Basic information of circular RNA
PanCircBase ID:
mmu_ciG6pc2_629
mm39_Chr. Position ID:
chr2|69056271|69056900|+
Gene name:
G6pc2
Transcript:
ENSMUST00000129288.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
629
Exon/intron sizes in nt:
629
Flanking exon/introns:
chr2:69056271-69056911
Splice length:
629
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGTAGCTGAATCAAGACACGAACACAAATGCTACCCTGGGGGCTTCGGTGATTTAATAAGACCTTTCTCACGAGCCTCTGTGCACAGGATGACACAGTCTCTCTCAGTGGTGCAAATGGAGAACTGTGTGACGAGATGAGCTTTTCTGAGGTGTAACTGAAGTGGTCATCCAAACCATCATGTGCCTCCACCTTCTTTTGGGGGACCTCATAAGGACCCCAAACCCACAGCCAAGACACTCGGTTGCAGCTAAGTTCTTATTGCACGCATGGCTTAACCTCACCAGGTGGCAGCTTTGTATTGCTTAGTGTTTTGCCTGCAAAATGAGTTGTCACTCAGTGTTTCTGACTTTTGATTTATCCTTCTTGGCCAGTGTAGGTAGTTGAAAGACTATGGGACTAATCTGTTTTCAGTTTATGTCTTAAAACACTTTAGGAAATTGTTAGAACCTGGGTGTCTTAAACCCTCCCAGGTGCAGAGAGGAGGGGTTTCTGAAGGGCTCAGGGTCACAAGCTCATGTCAGTATCTCTTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCAGAGAGGAGGGGTTTC
60.04
CAAAGCTGATGACCGGGAGA
59.75
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCAGAGAGGAGGGGTTTC
60.04
TGATGACCGGGAGAGTCTGA
59.67
143
PCR template sequence:
TAGGAAATTGTTAGAACCTGGGTGTCTTAAACCCTCCCAGGTGCAGAGAGGAGGGGTTTCTGAAGGGCTCAGGGTCACAAGCTCATGTCAGTATCTCTTTGTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-320-3p(1), mmu-miR-125b-5p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-125a-5p(1), mmu-miR-22-3p(1), mmu-miR-758-3p(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGTATCTCTTTGTAAATATAGCCT
siRNA sets
ATATTTACAAAGAGATACT,TATATTTACAAAGAGATAC,AGGCTATATTTACAAAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIG6PC2_629_ORF_1
0.621
-0.175160419
0.046737885
252
ORF sequence:
ATGTCAGTATCTCTTTGTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGTAGCTGAATCAAGACACGAACACAAATGCTACCCTGGGGGCTTCGGTGATTTAATAAGACCTTTCTCACGAGCCTCTGTGCACAGGATGACACAGTCTCTCTCAGTGGTGCAAATGGAGAACTGTGTGACGAGATGA
Peptide sequence:
MSVSLCKYSLLYSVVSSLHFTLPLCSVRLSRSSALVSEVAESRHEHKCYPGGFGDLIRPFSRASVHRMTQSLSVVQMENCVTR
IRESfinder result
IRESfinder score
0.767924349
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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