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mmu_ciG6pc2_629

Basic information of circular RNA
PanCircBase ID:

mmu_ciG6pc2_629

mm39_Chr. Position ID:

chr2|69056271|69056900|+

Gene name:

G6pc2

Transcript:

ENSMUST00000129288.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

4

Exon/intron offsets:

0

Genomic length:

629

Exon/intron sizes in nt:

629

Flanking exon/introns:

chr2:69056271-69056911

Splice length:

629

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGTAGCTGAATCAAGACACGAACACAAATGCTACCCTGGGGGCTTCGGTGATTTAATAAGACCTTTCTCACGAGCCTCTGTGCACAGGATGACACAGTCTCTCTCAGTGGTGCAAATGGAGAACTGTGTGACGAGATGAGCTTTTCTGAGGTGTAACTGAAGTGGTCATCCAAACCATCATGTGCCTCCACCTTCTTTTGGGGGACCTCATAAGGACCCCAAACCCACAGCCAAGACACTCGGTTGCAGCTAAGTTCTTATTGCACGCATGGCTTAACCTCACCAGGTGGCAGCTTTGTATTGCTTAGTGTTTTGCCTGCAAAATGAGTTGTCACTCAGTGTTTCTGACTTTTGATTTATCCTTCTTGGCCAGTGTAGGTAGTTGAAAGACTATGGGACTAATCTGTTTTCAGTTTATGTCTTAAAACACTTTAGGAAATTGTTAGAACCTGGGTGTCTTAAACCCTCCCAGGTGCAGAGAGGAGGGGTTTCTGAAGGGCTCAGGGTCACAAGCTCATGTCAGTATCTCTTT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTGCAGAGAGGAGGGGTTTC

60.04

CAAAGCTGATGACCGGGAGA

59.75

149

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GTGCAGAGAGGAGGGGTTTC

60.04

TGATGACCGGGAGAGTCTGA

59.67

143

PCR template sequence:

TAGGAAATTGTTAGAACCTGGGTGTCTTAAACCCTCCCAGGTGCAGAGAGGAGGGGTTTCTGAAGGGCTCAGGGTCACAAGCTCATGTCAGTATCTCTTTGTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

10

Interacting miRNAs (#binding sites)

mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-320-3p(1), mmu-miR-125b-5p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-125a-5p(1), mmu-miR-22-3p(1), mmu-miR-758-3p(1), mmu-miR-193b-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TCAGTATCTCTTTGTAAATATAGCCT

siRNA sets

ATATTTACAAAGAGATACT,TATATTTACAAAGAGATAC,AGGCTATATTTACAAAGAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIG6PC2_629_ORF_1

0.621

-0.175160419

0.046737885

252

ORF sequence:

ATGTCAGTATCTCTTTGTAAATATAGCCTCTTATATTCAGTAGTGTCATCACTTCACTTCACTTTGCCGCTTTGTAGCGTCAGACTCTCCCGGTCATCAGCTTTGGTTTCAGAGGTAGCTGAATCAAGACACGAACACAAATGCTACCCTGGGGGCTTCGGTGATTTAATAAGACCTTTCTCACGAGCCTCTGTGCACAGGATGACACAGTCTCTCTCAGTGGTGCAAATGGAGAACTGTGTGACGAGATGA

Peptide sequence:

MSVSLCKYSLLYSVVSSLHFTLPLCSVRLSRSSALVSEVAESRHEHKCYPGGFGDLIRPFSRASVHRMTQSLSVVQMENCVTR

IRESfinder result

IRESfinder score

0.767924349

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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