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mmu_ciG6pc2_1082

Basic information of circular RNA
PanCircBase ID:

mmu_ciG6pc2_1082

mm39_Chr. Position ID:

chr2|69053387|69054469|+

Gene name:

G6pc2

Transcript:

ENSMUST00000129288.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

3

Exon/intron offsets:

0

Genomic length:

1082

Exon/intron sizes in nt:

1082

Flanking exon/introns:

chr2:69053387-69056155

Splice length:

1082

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTCAGCTTCTGAAGCATTAGCAAGACAGCGTTGAGCATGCGAGGTCGTTAGTAAACATTCATTTGAATACATTTACAGTTTTTCAACAAAATGTGCTTATTCGATTCTTTCATAGCCCATGTAGCAGAATGAGTGGAAAATGAATATTCTGCTTCAAGATAATGTTCTAATTAGCTCACAGCTATTACCACTTTTATAATTGAGTTGGAAATGTTTATCTGATTAATGACTTGCTAAATACGCCTTTGGGATTCCAAAATTCAATTATTATTTTGGTACCTCATAAACAAGTCATCTAGACTTGCATAAGGAAACATTTTTGCATGCACATTCTCTTGCCTTAAGATTCACTTAAATTTTTAAGGATCATCGTAGCTCTTAAATGTATCCATTTATTTAGTAAAATTATGCTTCCTACTTTGAACACCCAATGCTTTTAAGATCAGTAAAATATATTCTAGCTGTTAAAGTCAATGCAGTCTTTTTAATTAAAACATTCTAGAGGCAAAATTAAATTTGAATGTTTGACCTACTGCTAACAGATCTCTTAATGTGTAGAAGCATGTAGAACTTTAAGATAACTCACTATTTGTAGAAAGTGATATTTTTCTTCTCTTCTCTTTTTGAAATTCATTGTAATTACAGTAATCCAAAACACCACTTTGTCACAAAGTATACAGTTTTGATTAGAAACAGAAATGAGCAAAAAAAAATCTTATGAAAATATTAAAATCTGAGCAATTTTAAAAGAAAAATAAAGCTGTATTGAGTGTCAGGAATCCTCAAATGTTATGCTGTTGACAAGCTGGCCAAAATTCAGTTACAGAAAGAAATTTGTCAATACTTTTCTTAAATATGGCATATAACCATGCCAGTATATAACATTTCTTTTAAAATAAAGAATTAAAAACACATTCTACTTCTAAAGAGTAGTCTTGAATAATGTAAGAAATAAGTCTGAGTGGGTCATTCTCCCCTCCAGTTAACAACCTTAAAGAACATTTTTATTCTAAAAAATATTCCTATTCCTTTGTGATGTTTGTAACAGCAATATTTTCTTCATTTCTAAAAATTTAGAGAGT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

AACAACCTTAAAGAACATTTTTATTCT

55.03

ACTAACGACCTCGCATGCTC

60.18

150

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

AACAACCTTAAAGAACATTTTTATTCT

55.03

CTAACGACCTCGCATGCTCA

60.18

149

PCR template sequence:

TTAACAACCTTAAAGAACATTTTTATTCTAAAAAATATTCCTATTCCTTTGTGATGTTTGTAACAGCAATATTTTCTTCATTTCTAAAAATTTAGAGAGTGTCAGCTTCTGAAGCATTAGCAAGACAGCGTTGAGCATGCGAGGTCGTTAGTAAACATTCATTTGAATACATTTACAGTTTTTCAACAAAATGTGCTTAT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

6

Interacting miRNAs (#binding sites)

mmu-miR-137-3p(1), mmu-miR-221-3p(1), mmu-miR-31-5p(1), mmu-miR-222-3p(1), mmu-miR-30d-5p(1), mmu-miR-574-5p(1)

siRNAs for circular RNA silencing
Junction sequence

AAATTTAGAGAGTGTCAGCTTCTGAA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.92125136

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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