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mmu_ciEro1b_4224
Basic information of circular RNA
PanCircBase ID:
mmu_ciEro1b_4224
mm39_Chr. Position ID:
chr13|12589820|12594044|+
Gene name:
Ero1b
Transcript:
ENSMUST00000221560.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
4224
Exon/intron sizes in nt:
4224
Flanking exon/introns:
chr13:12589820-12594056
Splice length:
4224
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGTGGCAATTTTTAATTCTGTTAATCCAAAGTTTTCTATATAGTTGTCTGCAAATGTGACTTCTTTTATTATGTTTTTCAAAATCTTTGTAAGAAATATCATTGTGAAAAGCTTGTTTACAACACACACCAAGAATCAGAACTCTTCTTCTTATGTTTGGAAATTCTTCCTCTTTCAGTTTCAGTTTTGAGGTGACGATTCCTCCTGCCCACACGGACTTTGTCAGCCTCTGTGAGCACAGGTTATGTGCTCTGGACCTCACTGGCAGGCTGCAGCGGGGCACTGGGCTGGTATAATGGGCGTATCTTAAACACCACAGAGTAATAGGAGGTCACTGGTGCTCTTTGTGGGGACTGCAAACTAAGTCATGCTACAAGTAGAACATGCCTCCACCGTTAGTCTGTGGACTCCGTTTTTTAACCTAGCATGAGTCCTGGGCCTTCTAAAACATCAGTGGAAAGGCAGGAACGGTATTGGATGTACGTCAACAATTAGAACGAAAAAAAATGTAATTTTATATTTTTAAGGAGACAGTACTAAGGGGGTAGCCTAGTGAGTTAAAACAATTATTCATGTCTATTCATTGTACAAGGCAGTGGGCGACATGGTGGCCTCTTTTGGTTCAAGTCCATCCTGTGCTTTGACCATGTTCACTCTCCGTTTCTTCAGTCCCTCCCCTCTGTGTGTTGTACAGTCTATTTTATAGCTATTTTTTAAAGCTATGCTTGGCTTTCCCTTAATCTTGTTCTTGCTCTTACGATTTGACTGGTAGATAATTTGGTTTTTATTTTAATTTGTTCTAAATTTTAAACCTGGTTTCTTTTTTAATCATGACTCTGAATAGATTATCTTAGTTCTTTAAATATTTTTGAGGGTGAAATTTTTAGTTTTTGGCAATTGTAGATTATCCAAGAAGATAAATTAGAAAATTACAAATTGGGACTCCATCCCTTTGACTACAGATACAACGAATATCCAGAAAGCAGTAAATGTTTCTTCTCTGTACAGAATCTGTTGAGAATGCAGCTGGTAGATTTATTTCAAAGATAAATCTTTACCTAAAACTGTGGCTGATCTTTGGGTATTTTTCATATAAATAAGATCGGTGCTCATGAGTTCCTGCATGACAGGAGTGGCTGTGTGTATAGACGTGTCCTTCATAATATTAGATAAACCAAAAAGAAGTTGTTACTTCAATGGTTTGAAAATGGTTGAACATGTCAGTTTCTTATGGTCCAGTCTAATCCAGTGGAAACTTTGTGTTGCTGTCAGGCACTGTGCTTTCATGTGGGGTCCAGGTACCATTTCCTCTGACTCTGTCCCAACCCTCTCTCAGCACATCTCTCTGTGGGACTGCATCTTCTATTTCTGTATTCACATAAGGCCTGGCTCATTGAAGAATTGAACTTTTAGATGAATGGATATCTCATTTAATCTTCATGTGAAATTTTTGTTCCTATCTACATTTTTAAATTGAAGAAATAATAGGCCTGGGTATCCTAAGTAGGATACTTTTGTTGACTAAAAAAACCCGTAAGTTTAAGAGAGAAGATAATTGAGACGTTTACAAACTTCAAAGACAAAGTGAAGTGGTACACATCAGAAGGATGAGAGAAGAGCAGGCGCGGATGGGCCTTCTCTTCATGGTAACACCAGAACTGCAGAGGTGGGTGGTTGACTGAGAGTAACTAGGCAAGCACTGTTACCCTGAGCTGTAGCATCAGCCCTTCAGGCCAAATGAGCTATTTTCCCATCTTGTTCTTGTTCTTTATAAAAGAGTCAAATTTCTGGAGGAGTGAATCATGACTGGTCCAGGAAGCCATGTGCTACCTTTACCAAAAGAAGAAAAGATACCTTGGGTGGCAGTGCTATTCCAAGGAGCATGAGGGATCATTGTTTCTGAAAGGAAATTAGAAGGCTGTTACAGGAGAAGGGAAAATGAGAGCTAAGGCAAAAATAACAGTGTGGCCAATCAACATAGACTTGTTTAGAAATGCTTTAACACGATAAGGTTGTTCCATATATGACCAATCCATGCCTGTCTCCCAAGAGGGTGTAGCCCTGATCTTGCTGATGCCTTCATCTCAGAAACAGGCAGGGATTTGAGAGAAAGATGCTCTGCTTCTAGTTCTGCCTGCCTGAGAGATAAATAGAAACATTTATCCATTCACAGTATGTTAACTAGTGAGAATCTAAGAGAAATTGATTTCAAACACACATATACAATCTAGGAATTTGGGGAACAAGGCGCTGCTGGAGGTGGAAGTAAGCTCCCACCTTTGCAGCTAGGCAATTGATTGGTCACACCGATGACCTCTAGTCTTGTACTGTGGATTCAGCAGTGAGTAAGACAAAGCTTGCGTTCTGCTTAGGGAGAAAAACAATAAAGAAGTCGATATTGAACTGTATTGCCAGATGTAAGGTGGTTACTATAGAGAAAATAAAGCAGGAGCAATGTAGATGGCGTGAGGATAAGGAATACAGAGATAGGTAGCCTTGCTTTGATGAAGTGATCAGGAAAGGATGGTGTTCATGCTAATGTGATATTTGAGCAAAGACCTTTCTAATGTGAAAGTATAAGTGGTGGGGAGATCTGGGGAAAGCACTTCAGGGAAGTTGAGTGAATAGATTTAAGAGTTTAGACACAGAAGTAAGCTTCGTATATTTGAGGAACTAGAAGGCAACTTAGGCCACAGAAAGCAGGTGAATGAGAAGGAGGATTGGAGAGAGAGGTAGGGACCAGCTTTAAAAACGTGTTTCTAGCTGCATATGTAGCAGAAGATAGCCTAGTCGGCCATCAGTGGAAAGAGAGGCCCATCGGTCTTGCAAACTTTATACGCCTCAGTACAGGGGAACGCCAGGGCCAAGAAGCGGGAGGGGGTGGGAGGGTATGGGGGACTTTTGGGATAGCATTGGAAATGTAAATGAAGAAAATACCTAAAAAAAAAAAAAGTAATGTAAAAAACAAAAACCAAAACCGTGAACGTGAACACATGGCACACACACACAGACATGATACAGAAATATACTGAGGGTTGGTTACATTTGAAGTACTACTTTAGAGTACTAGTCTAGTCAGGGTCTGCTGCTGTCAAGAGTGAGGTCGAGGAACAGTAATTCAGACAAAGAGAATGGGCAGTATAGAGATAGGCTTTGGGGCTTACAGAGCCCTGTATATCAAGTAAAAATGCAGTGTGATAGATGAGGTAAGAGAGGCAGGTGACAATTGTTACATAGTGTGTGTAGAGCTGCTGCAGGGATAGAGAGCTGAAAATGCACAGGACGGTTCTGAGCAGTCATGGGAAAGGTCTGCCTTGGTTTTGATGTAACTGTTGCTGAGTGCAATAGGTGGGGGCGAAGGAGGGAAACTATTGCAGACCCTTGGGCAAGAGACTCGATGGTGATGGAGGTGGTAGAAAGTAGCAAGGGAGGAAACAGAAAACTGTCTGGGTTTTTAGCTTGGGCAATACATCTGTAAGTCGTGTGGGATTATCAACTTCCTGTTGTATGAAGACTATGGGAACTGGTGTTTACCATGTATGTGACCTCTACACGTATTGTCCATTCTTTTTCCACCAAAAATGGTGGAACTGGTGGTTCTTCATTTTTAGCACCATCAAAGCTCTCCATTGCTGTAGCCAAATGCACACTTCCCCACCTTTCATCTTCCTAAAACATGGTCTCCTCAGAGGCTCCATCCATCAGCACTGTGACTACAAAACTCTAGGCTCTCCTTTGGAACACACACTACAATTTGCAATGAAATAAACATGTGAGTACTTGATTTTTCTTTAGGATATTGTAAGTCCTAGAAGAGAAGGCTCTCGTTGGCCTTGAGCCTAGTCTGGTGTCTGACAGCTAGCCAGTGCTTAGCACATACTAAGGTCGCGCTGAAGCCCTAAGGCTAAGGTGGTCGTTGGTGTTTGGGGAATGAGTATTAAAGTCATTCGGAAATGCTGCCCAGTGACAACTGTGCTTCAAACAAGCAGTGAACTTGACTGCTGTCCCTCTTTTGCAATGACAGAGAACCTATGGCCATTAAAAGCTAACACTCCGTCCAAGTGCCTGCATAGCCGTAGTCACTTACTGTCTGCACCATGTACACGCTGCAGGATCAAGCAGCTTCCCCCTCCCCATCACTACTGTGTCACTGTGCCCAACACAGTGACATCTGCAAACTGTCTGTAATTACCATGCCTGATCACTAATGTTGATTTGTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCCCCCTCCCCATCACTA
60.03
AGAAGTCACATTTGCAGACAACT
58.8
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCTCCCCATCACTACTGT
60.33
AGAAGTCACATTTGCAGACAACT
58.8
162
PCR template sequence:
AGCTTCCCCCTCCCCATCACTACTGTGTCACTGTGCCCAACACAGTGACATCTGCAAACTGTCTGTAATTACCATGCCTGATCACTAATGTTGATTTGTTGTAAGGTGGCAATTTTTAATTCTGTTAATCCAAAGTTTTCTATATAGTTGTCTGCAAATGTGACTTCTTTTATTATGTTTTTCAAAATCTTTGTAAGAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
39
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(3), mmu-miR-23a-3p(3), mmu-miR-335-5p(2), mmu-miR-340-5p(2), mmu-miR-1187(2), mmu-miR-7a-1-3p(2), mmu-miR-15a-5p(1), mmu-miR-32-5p(1), mmu-miR-103-3p(1), mmu-miR-221-3p(1), mmu-miR-26a-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-30c-5p(1), mmu-miR-328-3p(1), mmu-miR-222-3p(1), mmu-miR-320-3p(1), mmu-miR-143-3p(1), mmu-miR-25-3p(1), mmu-miR-138-5p(1), mmu-miR-339-5p(1), mmu-miR-181b-5p(1), mmu-miR-214-3p(1), mmu-miR-128-3p(1), mmu-miR-433-3p(1), mmu-miR-676-3p(1), mmu-miR-411-3p(1), mmu-miR-324-3p(1), mmu-miR-107-3p(1), mmu-miR-384-3p(1), mmu-miR-466a-5p(1), mmu-miR-1198-5p(1), mmu-miR-466p-5p(1), mmu-miR-207(1), mmu-miR-3087-3p(1), mmu-miR-3065-5p(1), mmu-miR-1904(1), mmu-miR-296-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGTTGATTTGTTGTAAGGTGGCAAT
siRNA sets
ATTGCCACCTTACAACAAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.765763327
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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