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mmu_ciDnajb12_1686
Basic information of circular RNA
PanCircBase ID:
mmu_ciDnajb12_1686
mm39_Chr. Position ID:
chr10|59726807|59728493|+
Gene name:
Dnajb12
Transcript:
ENSMUST00000147914.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
1686
Exon/intron sizes in nt:
1686
Flanking exon/introns:
chr10:59726807-59728493
Splice length:
1686
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGCAGCCCTGGGCTGTTTGAGGTTTTGTTTGTTAGTTTGTATCCCTATGTAGACTGAGCTGGCCTAGAACTCAGAGATCCCTCTGCCTTCAAATACTGGGATTAAAGGTGTATACCACTATGTCCAGACAGCTTAGTGTTTTTATTCCTGTCTTAAAATACAGGGTCAAGCCTGGGCAGTGGTGGTGTACACCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGAATTTCTGAGTTCGAGGCCAACCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACTCTTGTCTTGAAAAAACAAAAAAGAAAAGTCTTTAAAAGAAGAAGAAAAAAAAATTCAGGGCCGAGGCAGCTTACTCAGGAAGTGGGGCTAGACGAATGTGGTCTGTAGTGTAATTGTACAAGACCTGTTCCCTGAGCCACCCCTAGCCATATAAACTAGCAGCTCTCACACAGTGCCCCTGCCTCCTCCTCATTTTCCGGCTTACAGTAGAGGAGCCAGAGGCGTTCCAGTGGCTTCTCCAGGGTCAGCAGCACCTAGGCCATGAGCAGACTCTTGACAACAGTCCCTGTACCGAGGGGCAGGGCACCGCATACCAGGAGGGCGCCTTCAGAACAGGCTACCAGCTTCCTATTTCCCACAGCCCACCTCAGGCCTGTACTAGGGACAGAGAGACTTGTGATCATACAGCCCCTTGATCTGTGCCGTGAATGTGTCACCTCTGGCCAGAATCCTAAGTCATTCTTTATGCGTTTAGAGCTCCATCTTCCCTCTCCCTGGTACTCATGTGCACTGGCTCCATGCCTGCCCTCTGGTGCTGGGCCTTGCGTTTTCTTGGTCCCTGGGTACTGGTCCCTCTGGAGAGTAGCGTAGTGACTGGGAGCCGTGTTGTGCAGTACTGAGTATTAGCCTGTGGCAAAGTGGAGAGGCCTTGTGAGATGGGGAAAGAAGATTCCATCCTCCCTCCCAGGGGCTCCCAGCTCTGAAACTAGTCTGCAGCCCACTGCTGTGAGCGAGGGGAGCGTAGGGGATGCAAGCCCTGGGAGGCTGTAGCTCTCTGCCCTGGGAGCTCTGGTGAGGAGGCCCCAGCGCCAGATAGAAAACAGCAGCTGAGGCCTGGGCCCTGGGGGCAGCAGACTCATCCTGGCCACACAGCAGACACACGGGTGTCTCCACCAAAAACCGGGGGGTGGGGGTGGGGTTGTGTCTTCCCCGCAGTGTCTGCCGCACTCCCGTTTACAACCAGGACCTCGTGGGTCGCACCATGACTGCAGGATTATATTACACTGTCTCCTTGGCACCCCACAAGATGAGCGTGTGCAAGGGGCATTTCTGTACCATCAGTGGCAGAGCTTGAGCAGGACACACCTGAAGCAGGTGTATAGGCTCATGCCCACAACCCCAGTGCAGAGGCAGGAGGATAAAGAATGGAAGGCAACCTGGGCAAGTTCCTGGACCATTCTGGCAGTTTCAGTTTAGGAGATGTAGCAGGGACCCTGACTCAAAAAAGGAAAGAGCATCCTCACAGCCCCGACTTTAGCCTTGCCTAGAGGTGCTCTGGATTGCCTGCCCAACAGGCAAACCCTCTACCCCTAAGACCCCACTTCCGCTGAGACCTGAAAGAAGCGAGCTCTTGGCTGTGCTAAGGCTGTTTCTCCCCTTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTCTACCCCTAAGACCCC
60.11
TCTCTGAGTTCTAGGCCAGCT
59.99
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTAAGACCCCACTTCCGCTG
60.11
TCTCTGAGTTCTAGGCCAGCT
59.99
155
PCR template sequence:
CTGCCCAACAGGCAAACCCTCTACCCCTAAGACCCCACTTCCGCTGAGACCTGAAAGAAGCGAGCTCTTGGCTGTGCTAAGGCTGTTTCTCCCCTTGCAGGTAAGGCAGCCCTGGGCTGTTTGAGGTTTTGTTTGTTAGTTTGTATCCCTATGTAGACTGAGCTGGCCTAGAACTCAGAGATCCCTCTGCCTTCAAATAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-181a-5p(1), mmu-miR-221-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-222-3p(1), mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-181b-5p(1), mmu-miR-24-3p(1), mmu-miR-382-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-324-3p(1), mmu-miR-705(1), mmu-miR-673-5p(1), mmu-miR-3087-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TCTCCCCTTGCAGGTAAGGCAGCCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.656224266
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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