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mmu_ciCul5_690

Basic information of circular RNA
PanCircBase ID:

mmu_ciCul5_690

mm39_Chr. Position ID:

chr9|53534244|53534934|-

Gene name:

Cul5

Transcript:

ENSMUST00000166367.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

14

Exon/intron offsets:

0

Genomic length:

690

Exon/intron sizes in nt:

690

Flanking exon/introns:

chr9:53534243-53534934

Splice length:

690

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTAGATGGGCTGTCAGTTTGGGTGTTAGTAGAAAGTGTTACTTAAGAGCTGGAGAGATGTTTCCGTGGTTACGAACTCTGCTTTTCCAGAAGATCTGGATTCAATTCCAGGCAACCACATAGCAGCTCACATATTCATAACTTCAATCCCAAATAGATTTGATGCCCTCTTCTGGCCTCTATAGGCATTTGTGGTGCATAGGCATTTATGCAAACAAAACACCCACACATGTAAAATAAAGTAAAAACAAAACAAAAAGCTAAGAACAAGAACAGTGGCTGTGTTTTCAGGACTGTAGTTTTCATCATTGTGTTGCTTACCAAACAGACCTCTTGACTGTAGAATCCAGTCTGATCCCCAAACAACCCTCTCTGTCTCCCTGCAAAAAGGAGGGCAAGAAGGGAGAAGAACCTTCTTTTAAGGCAACCTATAAGCATTCTCCTCACCATTTCTACTTCAAACACATTGGACAGAATGAAGTCACATGAGTGACCACATTGAGCTGCAGGGAAATCTGGGCGATGCATTGTATGTGTGCTAAGATTATTACCTCTGAGTAAGGAAGAATGGGTCTTGGGGAAAACTAGCAATCTCATTTTCCCATAGTATCTTATGTGCATTTACTAGGTATTGGAGTAAGCTTCATGAAGAAATGAGTTACTTATTAAAGCCCATTATCTATTACA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGTGCATTTACTAGGTATTGGAGT

57.69

CACGGAAACATCTCTCCAGCT

60.07

144

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGTGCATTTACTAGGTATTGGAGT

57.69

ACGGAAACATCTCTCCAGCTC

59.79

143

PCR template sequence:

GCAATCTCATTTTCCCATAGTATCTTATGTGCATTTACTAGGTATTGGAGTAAGCTTCATGAAGAAATGAGTTACTTATTAAAGCCCATTATCTATTACAGTAAGTAGATGGGCTGTCAGTTTGGGTGTTAGTAGAAAGTGTTACTTAAGAGCTGGAGAGATGTTTCCGTGGTTACGAACTCTGCTTTTCCAGAAGATCT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

5

Interacting miRNAs (#binding sites)

mmu-miR-139-5p(1), mmu-miR-1187(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-296-3p(1)

siRNAs for circular RNA silencing
Junction sequence

ATTATCTATTACAGTAAGTAGATGGG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.789359062

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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