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mmu_ciCtbp1_641
Basic information of circular RNA
PanCircBase ID:
mmu_ciCtbp1_641
mm39_Chr. Position ID:
chr5|33407073|33407714|-
Gene name:
Ctbp1
Transcript:
ENSMUST00000079746.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
641
Exon/intron sizes in nt:
641
Flanking exon/introns:
chr5:33407058-33407714
Splice length:
641
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGCCCCTGTGCCCTAAGATGGACTATGCCCACCAATCTGATGACTATGACGTGGGAGACCATGTATTTGAGCACTGCCCTCTTGCTGGCTGGGTTAGGGGAGGGTTTGCTGTCTGCTCTCAGGAGGGGACAAGATCCTCAAGAATCTGTCTAGCCTTGGCCATAGTAATGGGCTGGGCTTAGACTTGGCTTATTTTCAGTTTGTCTCTTGATGTCCATGATGGTCCCATGGTCCCCAGACTCAGCGGGGCCAAGGCATACACTAGCTTGGCTTCGCATGCAGCCTCTAGTCCAGTCTGGGCTGACTTCCTTCTTGGGGCTCCAGCAGCTGACAAAGCCCATCAAGGGATAGCAGACCAGTTGAGTGATTAGCCACTGAAGAACACAGTGGAGTCTGTCTGAGAGCCCTCATTCCTAGGGTTTGTTCTGCTGGGCAGGGCTGTGCCAGCACCACAGCAGGGCTCTTCCTCTAAGGCTCAGAATCCCCTCTACCCCTTACTGTTAAGACCTTGGGGTAGGTTTGCTAGTTCTGCAGTTCCACTTTTTCAGATGCCTTGGGAAAGGCCAGGGGCCCTCCCATCCCAGGGCTGGTTGCCTTCTGCACCTCCCAGACTTGGGTGCTCAGATCACTCTGACTGGGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTGCACCTCCCAGACTTG
59.6
CTAACCCAGCCAGCAAGAGG
60.39
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTGCACCTCCCAGACTTG
59.6
CCTAACCCAGCCAGCAAGAG
60.39
144
PCR template sequence:
TTTTTCAGATGCCTTGGGAAAGGCCAGGGGCCCTCCCATCCCAGGGCTGGTTGCCTTCTGCACCTCCCAGACTTGGGTGCTCAGATCACTCTGACTGGGTGTGCCCCTGTGCCCTAAGATGGACTATGCCCACCAATCTGATGACTATGACGTGGGAGACCATGTATTTGAGCACTGCCCTCTTGCTGGCTGGGTTAGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-34a-5p(1), mmu-miR-370-3p(1), mmu-miR-34b-5p(1), mmu-miR-680(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCTGACTGGGTGTGCCCCTGTGCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICTBP1_641_ORF_1
0.6174
-0.126458927
0.053820794
246
ORF sequence:
ATGCCTTGGGAAAGGCCAGGGGCCCTCCCATCCCAGGGCTGGTTGCCTTCTGCACCTCCCAGACTTGGGTGCTCAGATCACTCTGACTGGGTGTGCCCCTGTGCCCTAAGATGGACTATGCCCACCAATCTGATGACTATGACGTGGGAGACCATGTATTTGAGCACTGCCCTCTTGCTGGCTGGGTTAGGGGAGGGTTTGCTGTCTGCTCTCAGGAGGGGACAAGATCCTCAAGAATCTGTCTAG
Peptide sequence:
MPWERPGALPSQGWLPSAPPRLGCSDHSDWVCPCALRWTMPTNLMTMTWETMYLSTALLLAGLGEGLLSALRRGQDPQESV
IRESfinder result
IRESfinder score
0.630463675
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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