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mmu_ciCrebbp_421
Basic information of circular RNA
PanCircBase ID:
mmu_ciCrebbp_421
mm39_Chr. Position ID:
chr16|3942078|3942499|-
Gene name:
Crebbp
Transcript:
ENSMUST00000205685.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
421
Exon/intron sizes in nt:
421
Flanking exon/introns:
chr16:3937720-3942499
Splice length:
421
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGAACTGCATTTCTCTCTTCCAGTCCTTAGCTTGTTTGCTTAGTGCTCTTTTAGTCTTTGGAGTATATTTTTAAGACTACAGAGTTCTGTTCTCCAAAATCTGCACTTTGAAAGCTCTTTGTATGATCTTGCCTTATGGGAAGAATCTTTTATTTTTGTTTCTTTTTTGGTGTCTCCCAAGTTTTTCAAGACAGGCTTATTTCTCTGTGCACCCCTGGCTAATCTGGAGCTCACTCTGTAGACCAGGCTAGCTTCAAATTCAGAGATCCTCTGCCGCTGCCGCCGCCCACCTTCCCCTCACCACTGCCCAGCAGAGGGGAGATATCTTAAAAGGGTGTGCTGGCAAGTGTCTTTTAGATTCAATGTGTTTGTGTGTGTGTATGTGTATTTATTTTTAATTACATTTTCCTCCTTATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAAAGGGTGTGCTGGCAAGT
60.69
AGCACTAAGCAAACAAGCTAAGG
59.5
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTAAAAGGGTGTGCTGGCA
60.13
GCACTAAGCAAACAAGCTAAGGA
59.5
143
PCR template sequence:
GAGATATCTTAAAAGGGTGTGCTGGCAAGTGTCTTTTAGATTCAATGTGTTTGTGTGTGTGTATGTGTATTTATTTTTAATTACATTTTCCTCCTTATAGGTAAGAACTGCATTTCTCTCTTCCAGTCCTTAGCTTGTTTGCTTAGTGCTCTTTTAGTCTTTGGAGTATATTTTTAAGACTACAGAGTTCTGTTCTCCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-31-5p(1), mmu-miR-106b-5p(1), mmu-miR-335-5p(1), mmu-miR-298-5p(1), mmu-miR-185-5p(1), mmu-miR-127-5p(1), mmu-miR-466d-3p(1), mmu-miR-539-5p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCCTCCTTATAGGTAAGAACTGCAT
siRNA sets
GCAGTTCTTACCTATAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.9649725
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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