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mmu_ciCrebbp_286
Basic information of circular RNA
PanCircBase ID:
mmu_ciCrebbp_286
mm39_Chr. Position ID:
chr16|3914018|3914304|-
Gene name:
Crebbp
Transcript:
ENSMUST00000023165.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
22
Exon/intron offsets:
0
Genomic length:
286
Exon/intron sizes in nt:
286
Flanking exon/introns:
chr16:3913994-3914304
Splice length:
286
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGCGTGGTTTGTTGTAATATCTGGGAAGGGGGAGCAGCTGTACACAAGTCATGGGGGCGGTGGCTGTACTGTAGTCTTGTTCCTTCTCAGCAAGTATTCAGCAATTAAGGGAGGTGGCAGTTGGTTCTAAATTTAAGTCTTGTATTTGCCTGCTGTACAAATTGCAGACTCCAAGCCAGTCTGTCTCCTCACTCTTTTCACAATGATGTGATTTCAACTGCCAACAAAGTATTTCAGTGCTACATTTTACTGATTTGGCAACATACACTGTTGAGTATAATCTGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGTGATTTCAACTGCCAACA
59.36
GCCCCCATGACTTGTGTACA
59.96
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGTGATTTCAACTGCCAACA
59.36
GACTACAGTACAGCCACCGC
60.46
159
PCR template sequence:
TCCTCACTCTTTTCACAATGATGTGATTTCAACTGCCAACAAAGTATTTCAGTGCTACATTTTACTGATTTGGCAACATACACTGTTGAGTATAATCTGAGTGCGTGGTTTGTTGTAATATCTGGGAAGGGGGAGCAGCTGTACACAAGTCATGGGGGCGGTGGCTGTACTGTAGTCTTGTTCCTTCTCAGCAAGTATTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-101b-3p(1), mmu-miR-139-5p(1), mmu-miR-214-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGTATAATCTGAGTGCGTGGTTTGT
siRNA sets
ACGCACTCAGATTATACTC, AAACCACGCACTCAGATTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.834021418
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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