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mmu_ciCope_447
Basic information of circular RNA
PanCircBase ID:
mmu_ciCope_447
mm39_Chr. Position ID:
chr8|70760653|70761100|+
Gene name:
Cope
Transcript:
ENSMUST00000066469.14
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
447
Exon/intron sizes in nt:
447
Flanking exon/introns:
chr8:70760653-70761144
Splice length:
447
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGCCCAGATGGCAGCGTCAGCTTCCCCAAGGCCTGTTAGTTCTTGTGTCCCATACAAGTGACACACCAGCCTTAATGTAGCCTCTCCCTGGGGTCCTTTGCTGGGAACAAGGGTGTGGGACTCTGCCTGCTTCCCTCTGTAATACTGTGGGTGGACACTGCCTGTAGGGTAATGGGAGGCTCTGCACAGGACACGTGAGAAGCAAGCCAAGACCTAGCCCAGTTCTTTGGGGGATGGGGTGGGGTTGTTGTTGGGGGTGGAGTAGCAGCTCCCTAGGCTGTGGCAGGCCAGGAGCTTACCTGAGGTGGTACCACACAACAGAACTCTGTCTCCGGGTAGCATCTCTTGGATCTGTACCATGTCCAGGTGGGCCAGCCTTGTCTTCCCCGTATCAGCTGGACACATGCTAGTAGGAGAGTGTGCCAGGGTTCATCCCCAGGCTCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTTCCCCGTATCAGCTGGA
60.03
AAGGCTGGTGTGTCACTTGT
59.74
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTTCCCCGTATCAGCTGG
59.89
AAGGCTGGTGTGTCACTTGT
59.74
141
PCR template sequence:
CTTGGATCTGTACCATGTCCAGGTGGGCCAGCCTTGTCTTCCCCGTATCAGCTGGACACATGCTAGTAGGAGAGTGTGCCAGGGTTCATCCCCAGGCTCTGTGAGCCCAGATGGCAGCGTCAGCTTCCCCAAGGCCTGTTAGTTCTTGTGTCCCATACAAGTGACACACCAGCCTTAATGTAGCCTCTCCCTGGGGTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-221-3p(1), mmu-miR-34a-5p(1), mmu-miR-222-3p(1), mmu-miR-324-5p(1), mmu-miR-138-5p(1), mmu-miR-34b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCCCCAGGCTCTGTGAGCCCAGATG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICOPE_447_ORF_3
0.7368
-0.136400726
0.021837457
90
ORF sequence:
ATGTCCAGGTGGGCCAGCCTTGTCTTCCCCGTATCAGCTGGACACATGCTAGTAGGAGAGTGTGCCAGGGTTCATCCCCAGGCTCTGTGA
Peptide sequence:
MSRWASLVFPVSAGHMLVGECARVHPQAL
IRESfinder result
IRESfinder score
0.628437022
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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