top of page

mmu_ciAp3d1_1964

Basic information of circular RNA
PanCircBase ID:

mmu_ciAp3d1_1964

mm39_Chr. Position ID:

chr10|80563847|80565811|-

Gene name:

Ap3d1

Transcript:

ENSMUST00000020420.9

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

4

Exon/intron offsets:

0

Genomic length:

1964

Exon/intron sizes in nt:

1964

Flanking exon/introns:

chr10:80563823-80565811

Splice length:

1964

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAGGCCCTGATGCTTTCCTGCCACTCAAGTGAGCCACAAAAGATCAGCTAGCTGACAGTCCCACCTGCCTGGGGTAGCTTTTCAAAGCTTTCTCACTCATATCCCCTGTGTCTCAGCAGATGAGAGATCTGGTCTCAGCAAGCATGGGCCTTCACATTCTAGAGGGCTTCAGGCTGGAGTACATGCCCCTCCTCCCTTCCCTGCTCAATGCACACTGCTACAGCCTGTCCCTGTCCACACTGGGCTCCTGCCTGCCTGCTGGCTCCCAGGCCTCTAGATGGTCAGTGAGGGAGTGTGTGAGGGACTGAACATGAGGGAGTGGGTGTGAGTGTGAGGGAGGGAGTGTGTGAGAAAGTGTGAGGGAGGGAGTGTAAGGGAGGAAGTGTTGGAGTGGAAGTGTGAGGGAGTGAATGTGAGAGAGTGAAGGAGTGTGAAGGAGGGAGTGGATGAGTCCTCCTTGACACCATCCTGCTCCTGCCTACCTTGTGCTGAAAACCACAGGGTGCTGGCTGTTCTTTGTTTCAGCCCCTTTATAATTCCTGCCATAAAGTGCACCCCTCTGTTCTCAAATGCTGGAACAAGGGCAGGACTCCCTCAGTGTGAGGTGGCAGGTGTGTGTTATAGGCAACTGTCAGGTGGAAGAGGAATGAATATGGGAGCTACAGTAGCATAGCTGGGCTCATGGTGCTGGCTGAGGGCAGGGGTGAGGTGGGAGGTGCAGGGCTGTGTGGTGTTATGCTGCAAGCCACATGGGCAGTGTCTCTGGGTTATGGGGTCAGGCAGGAGGCCTGGGTGAGCCAGAGCTATTTGGAGGAGCAGATAACCTGAGTTGGCCAGGCCTTTGAGAAGAGCAAGGGTCTGAGTGATTAGGGCCCTCTGAAGGAGGCTCCTGGGTGCTATTCCTGCATGCATGAGCCTTATGAGAAGAAGCTGGACTTGAGTGGCCAGGCTCTGGGAGGACTGGACATGTGAGCTCTAGGAGGAAGGGGAGTGGGATACAGAGGTGAGCAGTTGGGTTGAGGCTGAGGCTGGCTCTAGCCACAACAGCATCTCACTGTCTGTATGCCTGCAGTGTGTGAGGCTGTGGGTCAGGCCTTCCTCTCTAAGACACAGGAGCTCAGCTTCATAAGAGAAGGTGTCCTACTACTGAGGATAGCAGTAACTTGCTGATGTCCTGGTTGGGACCTGGTATATGTGCAGCTGAGATCTCTGCCCACACAGCAGGGCACATGTCCCAGGCCTCTGCAGCCGCTGTGTCTCTAGACCAGCATGGGATCTGGGCATGGGATTCTCAAGGCCAAGCTCCAGGCCATCTGTCACCTCTGTGTGTCTTCCTGATGAGCTTTCCTAGTAGGGGGCAGTGCTTCCATTGATTCTACTGCTTTCCTTTCTGTCCCTTTGGGAGATGGCACTGCAAATTTTGGACACTGAGGGTAAAGGGCTGATCATAGCTTCTGATGCTGTTTAGCTTACCCTAAAGCTCACAACATTCTGTTAATAATCATGAGACCAGCACTCTGAGCATGCCCCATGTACAACATAACTGAAGTACACCCTCCTAGCATCAGGCACACTGCCTTAGAGTGGGGAGTCAGGGCACTGGGAAGAAGTTGCCTCAGTCTTCTGCCTTAGGCCCTATACATTAAAAGGATGAACTCCTTCTAATCGGCCCAGAGCATCTCAGATCAGAACAGCCAAAGGACTGTTCCTGGTATTATGAGGGAGTTGCTGAATAGAAAAGGTTCCTATAGCATTGCCAGGAACCAGGAAGTTGTCCAGGAGTTGTGACTTGCAAAGGAATCTCTGTCCCCAAGGAGCCCAGAGACCTGGTGGCTTCCACACAGAGTTTTTTGTGTTCTCTGCCTCTGACCTGTGTTCCAATTCTTGATGACAGTAGAGGAGCTGTGAGAGGCTCCCTCGTGCTGGGTTACAGACATACAGATAGTCCCAAGGGACTGCCACT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TAGAGGAGCTGTGAGAGGCT

59.37

TGAAAAGCTACCCCAGGCAG

59.96

154

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GTAGAGGAGCTGTGAGAGGC

59.26

TGAAAAGCTACCCCAGGCAG

59.96

155

PCR template sequence:

CTCTGACCTGTGTTCCAATTCTTGATGACAGTAGAGGAGCTGTGAGAGGCTCCCTCGTGCTGGGTTACAGACATACAGATAGTCCCAAGGGACTGCCACTGTAGGCCCTGATGCTTTCCTGCCACTCAAGTGAGCCACAAAAGATCAGCTAGCTGACAGTCCCACCTGCCTGGGGTAGCTTTTCAAAGCTTTCTCACTCA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

23

Interacting miRNAs (#binding sites)

mmu-miR-326-3p(2), mmu-miR-324-3p(2), mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-29b-3p(1), mmu-miR-709(1), mmu-miR-181b-5p(1), mmu-miR-214-3p(1), mmu-miR-382-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-370-3p(1), mmu-miR-541-5p(1), mmu-miR-340-5p(1), mmu-miR-34b-5p(1), mmu-miR-107-3p(1), mmu-miR-342-5p(1), mmu-miR-342-3p(1), mmu-miR-673-5p(1), mmu-miR-540-3p(1), mmu-miR-1904(1)

siRNAs for circular RNA silencing
Junction sequence

AGGGACTGCCACTGTAGGCCCTGATG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.563831102

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

bottom of page