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mmu_ciAnxa11_1045

Basic information of circular RNA
PanCircBase ID:

mmu_ciAnxa11_1045

mm39_Chr. Position ID:

chr14|25873322|25874367|+

Gene name:

Anxa11

Transcript:

ENSMUST00000022416.15

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

4

Exon/intron offsets:

0

Genomic length:

1045

Exon/intron sizes in nt:

1045

Flanking exon/introns:

chr14:25873322-25874367

Splice length:

1045

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTGCTTGTCCAAACTGCGTTCAAGCACAGATCTCTGGTCTAGCCAACAAGTTCTTAGAGGATTGAAGGGAAGGCTCAGGCCTCCAGCTACAACATACAAAAGAGCTCTAATTTCTCTTGTGTGCCCACCGAAGGCAAACACACAAGGTCTGGAAGCACAGCCCTGGTGGTTGCTCTTCCGTGAGATTACTCCAGAGGCTTCCAGCATCATGGCCTCATGCCCAGTAGGCCCCTTCTGTGACTTCCTAGACAAATGTGAAGGAGTCTCAGAATCCTCTCTGGATAGGGAAGATTGGCAGCTCCTCCAGGATTTATTTTGGCTCATTCTGGAGATCAGTTTGTGTTAGCAATGCCTGGAATTCATTGTCATGGTTACATGGCCAAACCAGCCAAGCACAGGGCTGGTGGTATGCGCTGGCTGCCTAGTTTTAGACTATTCTAGAGGGTCCATCACCTCCTTGCTTTCATTCCACTGCAACACTGCCCACTCTTCCCCACCCTGAGCGGCTGCCCACTGTTGCTCCCGTCTGTTCCCCATCATGCCCCTTATTGCGGTGATAACCAGCGTGTTCTAGCCACTAGAAAGGCCACTCACTAGGCTACAATAAGCCTGTTTGTAAACACCTGAGGGGAGCACACCTTAAAAGGAGGAAGGTTGATTAGGGCTCATGGTTTCCATCGATGGCTTCTTGGCTTTGTGACTGTGGGCCTGTGGTGGGCAGAACATTGTGGCAGAGAACACTTGGTAAACCAAAGCATAGCAGACTGGAAGTAAATAATGAAAGGGGCTCGGGCCCCAATATTCCCCTCAAGGACCTGCTCCGTCTCTAGTGACCTAATATCTTTCCAGCCGCTACTTCCTAAACACCACATTGACCCGTGAACCTTGGGGCTATTTGTCCAGTGCTTTTGATTTCCTGCTCCTTTAGGTACCTCAGTGCTTAGAGAAGGGGGTGTTTGTGGGATACGAAGAGGTAGAAGAGTCTTACACCTCGGGGCGGCATGGGTGGGTCATCTTACCTGTGGCTATTTGTCTGAGTAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GAAGGGGGTGTTTGTGGGAT

59.89

GCCTGAGCCTTCCCTTCAAT

60.03

180

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGGGTGTTTGTGGGATACGA

59.67

GCCTGAGCCTTCCCTTCAAT

60.03

176

PCR template sequence:

TAGAGAAGGGGGTGTTTGTGGGATACGAAGAGGTAGAAGAGTCTTACACCTCGGGGCGGCATGGGTGGGTCATCTTACCTGTGGCTATTTGTCTGAGTAGGTAAGTGCTTGTCCAAACTGCGTTCAAGCACAGATCTCTGGTCTAGCCAACAAGTTCTTAGAGGATTGAAGGGAAGGCTCAGGCCTCCAGCTACAACATA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

14

Interacting miRNAs (#binding sites)

mmu-miR-9-5p(1), mmu-miR-23b-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-216a-5p(1), mmu-miR-23a-3p(1), mmu-miR-22-3p(1), mmu-miR-483-5p(1), mmu-miR-218-5p(1), mmu-miR-539-5p(1), mmu-miR-335-3p(1), mmu-miR-669d-5p(1), mmu-miR-350-3p(1), mmu-miR-3061-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TTTGTCTGAGTAGGTAAGTGCTTGTC

siRNA sets

GACAAGCACTTACCTACTC

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.68112518

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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