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mmu_ciAmigo2_313
Basic information of circular RNA
PanCircBase ID:
mmu_ciAmigo2_313
mm39_Chr. Position ID:
chr15|97144508|97144821|-
Gene name:
Amigo2
Transcript:
ENSMUST00000053106.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
313
Exon/intron sizes in nt:
313
Flanking exon/introns:
chr15:97144478-97144821
Splice length:
313
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATTTCCGTTCAATTGTTTATGGGATGCGATTGTGTGTTTTTGTGTGTGTCGGTGGAGGGAGTTAAGGCAGAATGCATCCTTTAGAAACAAAAACACACAAAAAAGCCTGAGTTTCTCATGCTGAGACTGTCGCATTGAGAGAAGGTACCTGGAAATCTCACTGAGTTGGAGTAATCTAGGAAAAATGCATATAACAGGTTCTTTATTAAAAAGCCACCCCTGTTGGAGATGGAACCCATTTCAAAAGTTAATTGGAATGAAAGCAATTACGGAATAACTCTAACGGGTTAACTAGTGGCAGTTAACTTGTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGTTGGAGATGGAACCC
60.03
TAACTCCCTCCACCGACACA
60.18
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCTGTTGGAGATGGAACC
60.03
TAACTCCCTCCACCGACACA
60.18
161
PCR template sequence:
AGCCACCCCTGTTGGAGATGGAACCCATTTCAAAAGTTAATTGGAATGAAAGCAATTACGGAATAACTCTAACGGGTTAACTAGTGGCAGTTAACTTGTAGTATTTCCGTTCAATTGTTTATGGGATGCGATTGTGTGTTTTTGTGTGTGTCGGTGGAGGGAGTTAAGGCAGAATGCATCCTTTAGAAACAAAAACACAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(1), mmu-miR-384-3p(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGTTAACTTGTAGTATTTCCGTTCA
siRNA sets
AAATACTACAAGTTAACTG, GAACGGAAATACTACAAGT, AACGGAAATACTACAAGTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.814767422
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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