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mmu_ciAkap8_1028
Basic information of circular RNA
PanCircBase ID:
mmu_ciAkap8_1028
mm39_Chr. Position ID:
chr17|32528523|32529551|-
Gene name:
Akap8
Transcript:
ENSMUST00000002699.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
11
Exon/intron offsets:
0
Genomic length:
1028
Exon/intron sizes in nt:
1028
Flanking exon/introns:
chr17:32528523-32529551
Splice length:
1028
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTTGTGATCCAGTATCCAGGTACAGAACATGACTCCTGTAAGCTGGCTGCTCTTCCAGAGGACTCGGGGTTTGATTGTTATTACCACACGGCAGCTCACAGTCATCTGTGGCTCCAGTCCCAGGGAATCCAATGCCCTCTCTTGGCTTCTGTTCACATAGACATGAATACAGACAAAATACCAACACACCTAAAATAAATAGATTAAAAAAGAAGACAGAATGCAAAGGTTTATATAGTTCTATATATTGCAGTACTTAGCATGTATGAGGTCAGAGTTTGTTTAATCTCCATACCATGAAAAAACAGCCCAGGGCCTAAAGAAGTCCCTCAGAACTTAGGAACACTGACTGGTATCCTACGGTTTTTGAGCTCCAACATAGCAGCTCACAACTGTCTGTAACCCCAGTTTTAAGGGCTACGATGCCCTGTTTCTGGCCTCCTTGGGCACTGGGCGCATGTGGTGAGGATGCCCTGTTTCTGGCCTCCTTGGGCGCTGGGCGCATGTGGTGAGCAGACATATGTGCAAGCAAACTACTTTTTTTTTAATTAAACAGCCAAATGATGGCAGTCCGCACCTTTAATTCCCAGCACTAGGGAGGGGAAAGCAGGTGAGTTCAAGGACAACCTCTTCTACCGAGCAAGTCTCAGGATACCTAGGACTGCATAGTGAGACCCTGTCTTCAAAAACTAAACAAGAACAACAGTGCAGTGCTAGTTAAGGGCTACCAAAAATGTGAAGCCGCGTGCTGTGTAACAACATTAGTGGAAAGAAGCCCTGCTTGTGCTGAAATGAGAGCACAGCTGCGTTGATGGCCCATCAGGGATGTGAAGCAGCCTAATGGTTCTCTCCTGGTATAGCCACAGCTGGAAGGATGAGAAAGGCCAAAACAACATTTCTCACAGTGGAGCTGATCTCCACCTTCTCACCTTCTGTCCTAAAGATATCCTAGAGGGTAGAACAGGGAGAGTGTGGACCTTGCTGGAGAGGAGGGAGAAAGCTAATTGTTTGGTTTCCATTCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGGGAGAGTGTGGACCTT
60.1
AAACCCCGAGTCCTCTGGAA
60.47
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACAGGGAGAGTGTGGACCT
60.1
AAACCCCGAGTCCTCTGGAA
60.47
141
PCR template sequence:
CTCACCTTCTGTCCTAAAGATATCCTAGAGGGTAGAACAGGGAGAGTGTGGACCTTGCTGGAGAGGAGGGAGAAAGCTAATTGTTTGGTTTCCATTCCAGGTAAGTTGTGATCCAGTATCCAGGTACAGAACATGACTCCTGTAAGCTGGCTGCTCTTCCAGAGGACTCGGGGTTTGATTGTTATTACCACACGGCAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-185-5p(1), mmu-miR-22-3p(1), mmu-miR-467b-5p(1), mmu-miR-3087-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTTCCATTCCAGGTAAGTTGTGATC
siRNA sets
ACAACTTACCTGGAATGGA, CACAACTTACCTGGAATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIAKAP8_1028_ORF_1
0.6727
-0.073729248
0.050476127
195
ORF sequence:
ATGGTTCTCTCCTGGTATAGCCACAGCTGGAAGGATGAGAAAGGCCAAAACAACATTTCTCACAGTGGAGCTGATCTCCACCTTCTCACCTTCTGTCCTAAAGATATCCTAGAGGGTAGAACAGGGAGAGTGTGGACCTTGCTGGAGAGGAGGGAGAAAGCTAATTGTTTGGTTTCCATTCCAGGTAAGTTGTGA
Peptide sequence:
MVLSWYSHSWKDEKGQNNISHSGADLHLLTFCPKDILEGRTGRVWTLLERREKANCLVSIPGKL
IRESfinder result
IRESfinder score
0.67017538
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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