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mmu_ciAkap10_4285

Basic information of circular RNA
PanCircBase ID:

mmu_ciAkap10_4285

mm39_Chr. Position ID:

chr11|61763847|61768132|-

Gene name:

Akap10

Transcript:

ENSMUST00000102650.10

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

14

Exon/intron offsets:

0

Genomic length:

4285

Exon/intron sizes in nt:

4285

Flanking exon/introns:

chr11:61763847-61768132

Splice length:

4285

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGGGTTACTTCTATTCTGACCTAGCCTTTTGTTGATGTTGTTTGTTTGAGACAGGGTCACAATGTAGGCTGGGCTAGCCTCAAAGTCTTGTGGCCAGTTCACCTGGACCTTCTGATCTTCCCACTTCCACCTCCTAACTGCTGGAATTATAGGACGTGCCATCACAGCTGATTTGTACAGTGCTGAGGAAGGACCCTTGGGCTTTGTGAATGTTAGGTAAACACTTTAACAACTTAACTGTATCTCTGCCCTTGACCAGCTTTTGTAAAATAAATCAATAAGCACACACACACACACACACACACACACACACACACACACACACACACACTTTGAGTATTTAGTAATTAAAAAAAAAAAAAAGAGGAGCTGGAATTAGGACTCTGCAGTTAAGAGTGCATACTGCTCTTGCACAGGACCAGAATTTGGTTCCCTGTATCCACAGCCATCTCTAACACATAGAACTGATGCTTGTGGTCTCCTCAGATCCCTGCACTCTCACACACATACAACATTAGGATCCTTCTGTATCTCCCTCGGAAGAACTAACTGCTATAGGAAGTATCTTTCTCATCATAAGGCACTGCAAAGATGGTGAACTAAGTGCAAACTGTTTCTAGATCATGGATGTTGGGCATATGGGACTGTAATCCCTGAAAGAAGTAGGATTCAAGTGAGCCCTAGCCCACTGTTGAGAACTGGCTTCTGAAATAGAGAAGGCAAATCTGAACAGAGCCCAGGAGATTTGCTGAACTAAAAAATCAAAAGGAAAGTTTAGAGAGGGAAAGCTTGATGCAGTTATTTATAGCAGAAAAGAAGATACTGTCCAGAAATAAAAGGTACAGAGATACTCGAGGAAGGTCTCAAACCATTGGCCTAGCCAGAAACCGATCTGCATGGATGTGGAAGAAAATGCTTGTCTCAAGGGACAAACCCCAAAAGTTTGCCAAATGATGCCTGAAAGACAGGGCTAGGAGTAGTGTAGTTGATGTTAACACAAAGGATACAGAACAGTGGGGACTTGTGTCTTAATACTTGAGCTAAGTGAGCCCTAATAGACCTCAACAAAAGTAGTTTGGAAGGATCAGACTAACTTCTAAGTAACAACTGTATGCTAAAACTAAAGCTAGTCCTCTTTAATGGAACACAAGTAAAGATGGAAAGAGAGGGCTGAGTGACACAGTTGTCCTGAACTCCACAGCATGCTCTAGCAGATTCACCACCTGCACAGGGGCGTGCAGACTGAAAATTTAATATGGAATAACATTTTACAAGAATGTCAAGGCAAACAATTTAATGGGAAGAAATAGATAGGTTTTAAAAACTAAGATTCCTGAAATAAGAAATAATATTAGAGATGAAAACTAACCCTGAATGAAATCATTTGCATTTAGGAAATACAAGAAGAGGTTCTCTGAAAAGAATGTCTACTCCTCCATGAAGACAAACAGAAAAAGACTGGAAATATAAATTAAATAAATAAATAAATAAATAAGCAAGCAAGCAATTGGATGATGTGATACCTTCTAACAAACATTTGAGGCACAAATGCAGAAAAAAATACTTGAAAAAATATTTATTAAAAGTTCCAGATTTAATGAAAACTTTAATATATTTACAGAGAAGCAATATAAACAAAATACTAGAATACATAACATGAGTTCTGGGTTCCAGTAATAAAAAGGATATTTAAAATAGCTGGAGGTATATTACACATATAGATAAGAACAGCAATCATCCTGTTAAAACTTAAGTAAGCCAGACGATCCAGTGTTTTAAAATTGCCTAATACGTAAAAGTCAAACAAGAAGGTATCTCTTGATAAGTGAAGTTAAAATAAAGGGATTTTTATAAGCCAAACAAAACCTGAGAGAATTCAGTCCTAGTGACCGAACAACTGAAATCAATCCTAAGAAAATTCTTTTAAACCAGGTGTGGCAGTAGAACCTATAATTTTAGTTCTCAGGAACCTGAAGGTGGAGGATCATGAATTTAAAACCAACCTGTGCTTATATATAGGGAACTCAAAGAATGCCTGGCCACATAATAATACTGTCTAAAAAAAAACAAAACAAGCAATCCAACAAGAGTTTTTAATTAGTTTTTAATTGCAAATTTTTGACACTTCCTGGATGAATTTTCCAAGACAAAGTCTCTATAGCCCAGGCTATCCTTGAATTCATAATACTCTTCTAATTTCAGCTTTTTTAAGTATGAGGATTATATTCATAAGTCACCACATCCAACTGTGTTCATTTTTTGCAGGGCGGGGGGAGGTGTCAACTATTGCTATACTTTACATTCAAGCAAAAAGACATCTTGGGAAACTATATTCATAAGGGTCCTGTGCTTTATCAAGCACAGGGTCTCACGCATGCTAGATGAGTGGTCTTTCCTAAACTGTATGTATCCTGGCCTAGGAAAGTGTACTGTGACATCCTCAGCAGCTTGGATGTTTTGAATTTGATGCAGGTCCAACATTCATCTTGCCCTGCCAGATGTTAGAGAGAGAGAGACAGAGACAGGGACAGAGAGAATGACAGACAAAGAGAAAGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGAGAGAAAGAGAAAGAGAGAAAGAAGAGAGAGAGGAAAGGAAAGGAAAGGATAAAGGAAAGGAGAAAGGAAAGAAAAGAAAAGAAAAGAAAGAAAAAGAGAGAACCAAGCTCCTCAGGGCCTCCTGAATGCTCTACTGTGGAGCCGACCCTCCCAGCCTTTGGATGTGTTTTGTGAGGTCTTTCTTCCTTCAGTGATAGATCAGAGACACTGGGAGAGGAAAACCTCAGAAGACCTCCTTCCGATTCTCATTCCATGGCCATGAATGTGAGCAGACATGAGCCCATACAGATTATTTGGTCTGTGTAGCCAGTTTGCCACCTCTCTGTTTTGAATTCCTTGGATTCAATATATTTGTGCCTGGAGTGTATTTAGGCATTATCTCACATCATTACTGAATTTTTAAAGGATTTGTTGCTGAGTTCCAGGACAGCCAAGGCTACACAGAGAAAACTTGTCGCCAAAAACAAAAACAACAACAAAAAAGCAATCCTTATGTGTCTCCTGGGTTATTTAAGTTATGTAGTGTTGTCAAAGACAGTGTGTATTCAGTATCTGCACGTGTGTCTTTACCTTCTCAGATGAGTTCAGTAGAGTTGAGTGAGTCCTACAAGTAGAACCTCGTCAAAAAATAAAAATATCAGGCCGGAGAGATGGCTCATCAGTTAAGAGACCCCAGGTTCAGTTCCCAGCACCCATGTGACAGCTCACAACTGTTTGTAACTCCAGTTCCAAGGGATGCTTACACAGACATACATAATAGGTAAAACATCAATGTACATAATTTTCTTTTAAAAAATGAGAACATTGACAGACGTAGAGATCATTCAGTTGTTAAGTTCTTTGGAGTTTGATCCCTAGAACCCATATTAAAAAGCCAGATGCAGTGACATTTGTAACCTGGTGCTTACAAGCAGATTCCTAGGGTTCATTGTATCACTAAATTGCTGAGCTCCAGGGCAGTTAGAGTCCCTGTTTTAAAAAACACAAAGTAGGCTGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGAGGATTTCTGAGTTCAAGGCCAGCCTGGTCTATGGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAAAACCAAAGGGGAAAAAAAAACAACAAAAAAAAACCCCACAAAGTTTACAGCCCCTGAAGAATGGCACCCACAGTTCACCTTTGGTTTCTACACGATGTTCACACAAATGCACATGCAAATACATGAACACAAACATACAGAAAAGTGAATGGTCTTATTCTACTAACTCTGTCTAAACAGAAAAATGAATCCAGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCAGGTCCTGAGTGCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATTTGACGCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTATATACATAAAATAAACCTTTTAAAAAAAACTTCTTAGCTTCTTTAAGCTCCTGTGAACTCAGGAATATCTGAGGATAATTAATACTCTGCCCTAAGCCCTGACTGGAAGCACTGTATGTGCTGGGCATCTCTCTCATCTGTGCTGATAGTATGTCATCTACTACTGGAATGTACCTTACCTTTTACTTTTTCTTCTTTTTCCCTAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTGCTGGGCATCTCTCTCAT

59.53

AGCCCAGCCTACATTGTGAC

60.04

167

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

ATGTGCTGGGCATCTCTCTC

59.53

AGCCCAGCCTACATTGTGAC

60.04

169

PCR template sequence:

AAGCACTGTATGTGCTGGGCATCTCTCTCATCTGTGCTGATAGTATGTCATCTACTACTGGAATGTACCTTACCTTTTACTTTTTCTTCTTTTTCCCTAGGTAAGGGTTACTTCTATTCTGACCTAGCCTTTTGTTGATGTTGTTTGTTTGAGACAGGGTCACAATGTAGGCTGGGCTAGCCTCAAAGTCTTGTGGCCAG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

51

Interacting miRNAs (#binding sites)

mmu-miR-5101(5), mmu-miR-297a-5p(4), mmu-miR-1187(4), mmu-miR-181a-5p(2), mmu-miR-217-5p(2), mmu-miR-320-3p(2), mmu-miR-466a-5p(2), mmu-miR-466p-5p(2), mmu-miR-350-3p(2), mmu-miR-574-5p(2), mmu-miR-9-5p(1), mmu-miR-23b-3p(1), mmu-miR-199a-5p(1), mmu-miR-375-3p(1), mmu-miR-31-5p(1), mmu-miR-26a-5p(1), mmu-miR-92a-3p(1), mmu-miR-211-5p(1), mmu-miR-29b-3p(1), mmu-miR-335-5p(1), mmu-miR-29c-3p(1), mmu-miR-146a-5p(1), mmu-miR-18a-5p(1), mmu-miR-324-5p(1), mmu-miR-23a-3p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-miR-181b-5p(1), mmu-miR-351-5p(1), mmu-miR-26b-5p(1), mmu-miR-218-5p(1), mmu-miR-382-5p(1), mmu-miR-183-5p(1), mmu-miR-758-3p(1), mmu-miR-324-3p(1), mmu-miR-383-5p(1), mmu-miR-466d-3p(1), mmu-miR-690(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-669d-5p(1), mmu-miR-3087-3p(1), mmu-miR-3470b(1), mmu-miR-490-3p(1), mmu-miR-193b-3p(1), mmu-miR-146b-5p(1), mmu-miR-344-3p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TCTTTTTCCCTAGGTAAGGGTTACTT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.77856285

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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