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mmu_ciAcly_614
Basic information of circular RNA
PanCircBase ID:
mmu_ciAcly_614
mm39_Chr. Position ID:
chr11|100405173|100405787|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
9
Exon/intron offsets:
0
Genomic length:
614
Exon/intron sizes in nt:
614
Flanking exon/introns:
chr11:100405163-100405787
Splice length:
614
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGGCACAGACCCGAGTGCTGGGATGTACACACACCCGGAGAGCACAACATACGGAAGATAAGTGCTAGAATCACCGAGGCCGAGATCCTTTGTAATATAGGACCAGGCACTTATGTCAGGATTTTTTTTTTTCTCAGTGCTAACGGACTCTGGCAACACCACTCGGGCTCACATGGGGGCAAAGAGTGTATGAAATTCACCTCATATATGACTAGGGAGCCAGAGGAGAAAAAGGCTGCAGCATGCTTTTGCTGTGATGAATGTAAACTCAAGAGTTTAGTGATTCTTAGAGTTTACCAGACTTAGTGATGCCCAGTGTCCCTTCCCCACATTCCCAGTGTTCAGGGCTATACACACCAGGTCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACACTGACTGGATAATATGGGTAGTGGACACTTGAGCAAGCCGGGTGGGATTCTGGGAAGATCATCCTGCTCGCTCCCTGTGCCCCTTCCAGTAAGAGCATATTGGCTCAGAGGAGTGAGAACGTCCTCAGTTGGGTTTCTGGGGCCAGGCTCGAGTCTTCTATATCTCTAATGTTGTTGACTTTGCTTTTCACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCAGAGGAGTGAGAACG
59.83
TCTCGGCCTCGGTGATTCTA
60.11
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCAGAGGAGTGAGAACG
59.83
TCCGTATGTTGTGCTCTCCG
59.83
149
PCR template sequence:
GAGCATATTGGCTCAGAGGAGTGAGAACGTCCTCAGTTGGGTTTCTGGGGCCAGGCTCGAGTCTTCTATATCTCTAATGTTGTTGACTTTGCTTTTCACTGTGAGGCACAGACCCGAGTGCTGGGATGTACACACACCCGGAGAGCACAACATACGGAAGATAAGTGCTAGAATCACCGAGGCCGAGATCCTTTGTAATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-1a-3p(1), mmu-miR-206-3p(1), mmu-miR-181a-5p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-467b-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTGCTTTTCACTGTGAGGCACAGAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIACLY_614_ORF_1
0.6094
-0.108942144
0.101474281
333
ORF sequence:
ATGTTGTTGACTTTGCTTTTCACTGTGAGGCACAGACCCGAGTGCTGGGATGTACACACACCCGGAGAGCACAACATACGGAAGATAAGTGCTAGAATCACCGAGGCCGAGATCCTTTGTAATATAGGACCAGGCACTTATGTCAGGATTTTTTTTTTTCTCAGTGCTAACGGACTCTGGCAACACCACTCGGGCTCACATGGGGGCAAAGAGTGTATGAAATTCACCTCATATATGACTAGGGAGCCAGAGGAGAAAAAGGCTGCAGCATGCTTTTGCTGTGATGAATGTAAACTCAAGAGTTTAGTGATTCTTAGAGTTTACCAGACTTAG
Peptide sequence:
MLLTLLFTVRHRPECWDVHTPGEHNIRKISARITEAEILCNIGPGTYVRIFFFLSANGLWQHHSGSHGGKECMKFTSYMTREPEEKKAAACFCCDECKLKSLVILRVYQT
IRESfinder result
IRESfinder score
0.606659192
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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