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mmu_ci5330439M10Rik_85

Basic information of circular RNA
PanCircBase ID:

mmu_ci5330439M10Rik_85

mm39_Chr. Position ID:

chr10|117948560|117948645|+

Gene name:

5330439M10Rik

Transcript:

ENSMUST00000218667.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

1

Exon/intron offsets:

0

Genomic length:

85

Exon/intron sizes in nt:

85

Flanking exon/introns:

chr10:117948560-117959790

Splice length:

85

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAATTCTTCCACACTATCCTCTTCATCCTCGTAGCTGCAGTTCCAGATGGCATGGCCCCTGCGCCTTTGCTCTCTCTGACCTGG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TTGCTCTCTCTGACCTGGGT

60.18

ACTGCAGCTACGAGGATGAAG

59.86

60

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TTGCTCTCTCTGACCTGGGT

60.18

ACTGCAGCTACGAGGATGAA

58.81

60

PCR template sequence:

CCAGATGGCATGGCCCCTGCGCCTTTGCTCTCTCTGACCTGGGTAATTCTTCCACACTATCCTCTTCATCCTCGTAGCTGCAGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

NA

Interacting miRNAs (#binding sites)

NA

siRNAs for circular RNA silencing
Junction sequence

CTCTCTGACCTGGGTAATTCTTCCAC

siRNA sets

GAAGAATTACCCAGGTCAG, AGAATTACCCAGGTCAGAG, GAATTACCCAGGTCAGAGA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CI5330439M10RIK_85_ORF_1

0.6574

-0.142068932

0.022728841

117

ORF sequence:

ATGGCCCCTGCGCCTTTGCTCTCTCTGACCTGGGTAATTCTTCCACACTATCCTCTTCATCCTCGTAGCTGCAGTTCCAGATGGCATGGCCCCTGCGCCTTTGCTCTCTCTGACCTG

Peptide sequence:

MAPAPLLSLTWVILPHYPLHPRSCSSRWHGPCAFALSDL

IRESfinder result

IRESfinder score

0.710368801

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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